Molecular epidemiology of Bordetella pertussis in Cambodia determined by direct genotyping of clinical specimens
Molecular epidemiology of Bordetella pertussis in Cambodia determined by direct genotyping of clinical specimens
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DOI：
10.1016/j.ijid.2017.07.015
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发表时间：
2017-09-01
影响因子：
8.4
通讯作者：
Kamachi, Kazunari
中科院分区：
文献类型：
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作者：
Moriuchi, Takumi;Vichit, Ork;Kamachi, Kazunari
目的：本研究旨在确定的基因型循环百日咳博德特氏菌，百日咳的病原体，在柬埔寨的直接分子分型的临床标本。方法：DNA提取物从鼻咽拭子中获得82例百日咳患者在2008 - 2016年进行了多位点可变数目串联重复序列分析（MLVA）。B。结果：44份DNA提取物（54%）获得了完整的MLVA谱，并将其分为8种MLVA型（MT 18、MT 26、MT 27、MT 29、MT43、MT 72、MT 95和MT 200）。在发达国家常见的MT 27和MT 29是主要的菌株类型（共73%）。与毒力相关的等位基因主要是ptxP 3/ptxA 1/prn 2/fim 3A的组合（48%）。MT 27菌株在整个研究期间均被检出，而MT 29菌株仅在2014- 2016年被检出。在柬埔寨，全细胞百日咳疫苗（WCV）一直在持续使用，百日咳人口的死亡率与以前在使用无细胞百日咳疫苗的发达国家观察到的相似。循环B。柬埔寨的百日咳菌株与使用WCV的其他国家的百日咳菌株不同。(C)2017作者（S）由Elsevier Ltd代表国际传染病学会出版。
Objectives: This study sought to determine the genotypes of circulating Bordetella pertussis, the causative agent of pertussis, in Cambodia by direct molecular typing of clinical specimens.Methods: DNA extracts from nasopharyngeal swabs obtained from 82 pertussis patients in 20082016 were analyzed by multilocus variable-number tandem repeat analysis (MLVA). B. pertussis virulence-associated allelic genes (ptxA, prn, and fim3) and the pertussis toxin promoter ptxP were also investigated by DNA sequence-based typing.Results: Forty-four DNA extracts (54%) yielded a complete MLVA profile, and these were sorted into 8 MLVA types (MT18, MT26, MT27, MT29, MT43, MT72, MT95, and MT200). MT27 and MT29, which are common in developed countries, were the predominant strain types (total 73%). The predominant profile of virulence-associated allelic genes was the combination of ptxP3/ptxA1/prn2/fim3A (48%). MT27 strains were detected during the entire study period, whereas MT29 strains were only found in 2014-2016.Conclusions: The B. pertussis population in Cambodia, where a whole-cell pertussis vaccine (WCV) has been continuously used, resembled those observed previously in developed countries where acellular pertussis vaccines are used. Circulating B. pertussis strains in Cambodia were distinct from those in other countries using WCVs. (C) 2017 The Author(s). Published by Elsevier Ltd on behalf of International Society for Infectious Diseases.