Effects of aldosterone and potassium-sparing diuretics on electrical potential differences across the distal nephron.
Effects of aldosterone and potassium-sparing diuretics on electrical potential differences across the distal nephron.
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醛固酮和保钾利尿剂对远端肾单位电位差的影响。
DOI：
10.1172/jci108625
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发表时间：
1977
期刊：
影响因子：
--
通讯作者：
J. Kokko
中科院分区：
文献类型：
--
作者：
J. B. Gross;J. Kokko
我们以前已经表明，兔皮质集合小管的transstubular电位的变化与血浆盐皮质激素水平的变化一致，而远曲小管的电位是不变的这种变化。在目前的研究中，我们已经检查了在体外添加的d-醛固酮的影响，以及氨苯蝶啶和螺内酯的影响。d-醛固酮（0.2 μ M加入到灌注液中或1 μ M加入到浸浴介质中）在短的潜伏期后导致经前庭电位差（流明阴性）的显著刺激。d-醛固酮对正常或肾上腺切除家兔远曲小管电位差无影响。皮质集合小管的反应幅度和潜伏期的长度后，醛固酮显着的温度依赖性。氨苯蝶啶引起皮质集合小管电位差的逐渐但可逆的抑制，但对远端小管没有影响。螺内酯，当加入醛固酮之前，阻断皮质集合小管中的激素的电反应，并产生盐皮质激素刺激的小管的电位差的逐渐抑制。螺内酯对远端小管电位差无影响。我们的结论是：（a）醛固酮对远端肾单位电位的影响仅限于远曲小管，（B）对醛固酮的电反应和潜伏期是温度依赖性的，（c）对醛固酮的反应被螺内酯阻断，和（d）氨苯蝶啶抑制电位差只在皮质集合小管。
We have previously shown that the transtubular potential of the rabbit cortical collecting tubule varies in concert with changes in plasma mineralocorticoid levels, while the potential of the distal convoluted tubule is invariant with such changes. In the present studies we have examined the effects of in vitro addition of d-aldosterone to isolated tubules, as well as the effects of triamterene and spirolactone. d-Aldosterone (0.2 mum added to the perfusate or 1 muM added to the bathing medium) resulted in a marked stimulation of the transtubular potential difference (lumen-negative) after a short latent period. d-Aldosterone had no effect on the potential difference of distal convoluted tubules of intact or adrenalectomized rabbits. Both the magnitude of the response and the length of the latent period in the cortical collecting tubule after aldosterone were markedly temperature-dependent. Triamterene caused a gradual but reversible inhibition of the potential difference in the cortical collecting tubule but had no effect in the distal tubule. Spirolactone, when added before aldosterone, blocked the electrical response to the hormone in the cortical collecting tubule, and produced a gradual inhibition of the potential difference in mineralocorticoid-stimulated tubules. Spirolactone had no effect on the potential difference of the distal tubule. We conclude that (a) the influence of aldosterone on the potential across the distal nephron is restricted to the distal convoluted tubule, (b) the electrical response to aldosterone and the latent period are temperature-dependent, (c) the response to aldosterone is blocked by spirolactone, and (d) triamterene inhibits the potential difference only in the cortical collecting tubule.