EXPERIMENTAL-ANALYSIS OF BLOOD-VESSEL DEVELOPMENT IN THE AVIAN WING BUD
EXPERIMENTAL-ANALYSIS OF BLOOD-VESSEL DEVELOPMENT IN THE AVIAN WING BUD
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DOI：
10.1002/ar.1092310115
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发表时间：
1991-09-01
影响因子：
--
通讯作者：
NODEN, DM
中科院分区：
文献类型：
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作者：
FEINBERG, RN;NODEN, DM
在它出现后不久，鸟类的肢芽就被丰富的血管通道丛所填充。这种神经丛的形成是通过血管生成，特别是来自背主动脉或主静脉的分支的向内生长，以及肢体中胚层内源性血管母细胞的分化来实现的。然而，位于肢体表面外胚层正下方的间充质缺乏开放的血管。本研究的目的是确定外周肢体中胚层是否在肢体发育的各个阶段都缺乏血管母细胞或继发成血管细胞。在第9-10期鸡胚的头部，从16-26期的鹌鹑胚胎中移植核心或外周翼中胚层，通过全身输注墨水后有无开放通道来识别。3-5天后固定宿主，用识别内皮细胞及其前体的抗体处理切片，在任何阶段的肢体核心中胚层移植后，标记的内皮细胞均可嵌入到种植部位附近和远处的正常颅面血管中。在第16-21期移植肢体周围中胚层后，获得了相同的结果。然而，来自22期以上捐赠者的外周中胚层不含有内皮前体。因此，在附件发育初期，血管母细胞存在于肢芽的整个中胚层。在孵化的第四天，这些细胞从周围的中胚层中消失，要么是因为迁移，要么是因为退化。
Shortly after its appearance, the avian limb bud becomes populated by a rich plexus of vascular channels. Formation of this plexus occurs by angiogenesis, specifically the ingrowth of branches from the dorsal aorta or cardinal veins, and by differentiation of endogenous angioblasts within limb mesoderm. However, mesenchyme located immediately beneath the surface ectoderm of the limb is devoid of patent blood vessels. The objective of this research is to ascertain whether peripheral limb mesoderm lacks angioblasts at all stages or becomes avascular secondarily during limb development.Grafts of core or peripheral wing mesoderm, identified by the presence or absence of patent channels following systemic infusion with ink, were grafted from quail embryos at stages 16-26 into the head region of chick embryos at stage 9-10. Hosts were fixed 3-5 days later and sections treated with antibodies that recognize quail endothelial cells and their precursors.Labeled endothelial cells were found intercalated into normal craniofacial blood vessels both nearby and distant from the site of implantation following grafting of limb core mesoderm from any stage. Identical results were obtained following grafting of limb peripheral mesoderm at stages 16-21. However, peripheral mesoderm from donors older than stage 22 did not contain endothelial precursors. Thus at the onset of appendicular development angioblasts are present throughout the mesoderm of the limb bud. During the fourth day of incubation, these cells are lost from peripheral mesoderm, either through emigration or degeneration.