The light-hyperresponsive high pigment-2dg mutation of tomato:: alterations in the fruit metabolome
The light-hyperresponsive high pigment-2dg mutation of tomato:: alterations in the fruit metabolome
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DOI：
10.1111/j.1469-8137.2005.01362.x
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发表时间：
2005-05-01
期刊：
影响因子：
9.4
通讯作者：
Levin, I
中科院分区：
文献类型：
--
作者：
Bino, RJ;de Vos, CHR;Levin, I
光敏感高色素番茄果实的总体代谢变化（番茄）突变体植物和同基因非突变体（wt）对照植物进行了比较。靶向代谢物分析，以及大规模的基于非靶向质谱（MS）的代谢物分析，用于表现果实代谢物组成的差异。靶向高效液相色谱-光电二极管阵列检测（HPLC-PDA）代谢产物分析表明，hp-2（dg）果实中类异戊二烯和酚类化合物含量较高。非靶向GC-MS分析的红色水果产生25种挥发性化合物，表现出1.5倍的基因型之间的差异。高效液相色谱-高分辨四极杆飞行时间质谱联用分析红色水果结果表明，hp-2（dg）果实在ESI阳性和ESI阴性模式下分别产生6168个和5401个质量信号，其中142个和303个质量信号在基因型间存在2倍的差异。hp-2（dg）果实的特征是大量代谢产物的产生，其中几种已知具有抗氧化或光保护活性。这些代谢物现在可能更密切地涉及植物响应和管理光胁迫的资源。hp-1和hp-2突变体植物果实中代谢改变的相似性有助于我们理解hp突变如何影响细胞过程。
Overall metabolic modifications between fruit of light-hyperresponsive high-pigment (hp) tomato (Lycopersicon esculentum) mutant plants and isogenic nonmutant (wt) control plants were compared.Targeted metabolite analyses, as well as large-scale nontargeted mass spectrometry (MS)-based metabolite profiling, were used to phenotype the differences in fruit metabolite composition.Targeted high-performance liquid chromatography with photodiode array detection (HPLC-PDA) metabolite analyses showed higher levels of isoprenoids and phenolic compounds in hp-2(dg) fruit. Nontargeted GC-MS profiling of red fruits produced 25 volatile compounds that showed a 1.5-fold difference between the genotypes. Analyses of red fruits using HPLC coupled to high-resolution quadrupole time-of-flight mass spectrometry (LC-QTOF-MS) in both ESI-positive and ESI-negative mode generated, respectively, 6168 and 5401 mass signals, of which 142 and 303 showed a twofold difference between the genotypes.hp-2(dg) fruits are characterized by overproduction of many metabolites, several of which are known for their antioxidant or photoprotective activities. These metabolites may now be more closely implicated as resources recruited by plants to respond to and manage light stress. The similarity in metabolic alterations in fruits of hp-1 and hp-2 mutant plants helps us to understand how hp mutations affect cellular processes.