Effect of moxifloxacin on secretion of cytokines by human monocytes stimulated with lipopolysaccharide
Effect of moxifloxacin on secretion of cytokines by human monocytes stimulated with lipopolysaccharide
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DOI：
10.1046/j.1469-0691.2002.00374.x
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发表时间：
2002-01-01
影响因子：
14.2
通讯作者：
Remington, JS
中科院分区：
文献类型：
--
作者：
Araujo, FG;Slifer, TL;Remington, JS
目的探讨莫西沙星对脂多糖（LPS）和泛素酶刺激人单核细胞分泌细胞因子的影响。方法采集10例健康志愿供体的单核细胞，分别用LPS或Pansorbin刺激，并分别暴露于或不暴露于不同浓度的氟喹诺酮类抗生素莫西沙星。在3、6和24 h，采用酶联免疫吸附法测定单核细胞培养上清液中白细胞介素-1 α (il -1 α)、il -1 β、IL-6、IL-10、IL-12 （p70）和肿瘤坏死因子- α (tnf - α)的含量。结果LPS或泛素酶刺激人单核细胞可显著增加所检测的每种细胞因子的分泌。莫西沙星治疗lps刺激的单核细胞显著抑制（P < 0.01） 10个供体单核细胞分泌il -1 α；10个供体中有6个单核细胞的tnf - α分泌明显受到抑制（P < 0.01）。总体上有抑制IL-6、IL-10、IL-12分泌的趋势（p70），但抑制效果无统计学意义。莫西沙星对泛血素刺激的单核细胞分泌细胞因子无明显抑制作用。结论莫西沙星通过改变人单核细胞il -1 α和tnf - α的分泌，具有免疫调节作用。
Objective To determine the effect of moxifloxacin on secretion of cytokines by human monocytes stimulated with lipopolysaccharide (LPS) or Pansorbin.Methods Monocytes obtained from 10 healthy volunteer donors were stimulated with LPS or Pansorbin and exposed or not to different concentrations of the fluoroquinolone antibiotic moxifloxacin. At 3, 6 and 24 h, the amounts of interleukin-1alpha (IL-1alpha), IL-1beta, IL-6, IL-10, IL-12 (p70) and tumour necrosis factor-alpha (TNF-alpha) were measured in the supernatants of the monocyte cultures using enzyme-linked immunosorbent assay.Results Stimulation of human monocytes with either LPS or Pansorbin resulted in a significant increase in secretion of each of the cytokines examined. Treatment of LPS-stimulated monocytes with moxifloxacin significantly inhibited (P < 0.01) secretion of IL-1alpha by monocytes of each of 10 human donors; the secretion of TNF-alpha was significantly inhibited (P < 0.01) in monocytes from six of 10 donors. In general there was a trend towards inhibition of secretion of IL-6, IL-10 and IL-12 (p70), but the inhibitory effect was not statistically significant. Secretion of cytokines by Pansorbin-stimulated monocytes was not significantly inhibited by moxifloxacin.Conclusions Moxifloxacin has immunomodulatory activity through its capacity to alter the secretion of IL-1alpha and TNF-alpha by human monocytes.