Experimental subarachnoid hemorrhage causes early and long-lasting microarterial constriction and microthrombosis: an in-vivo microscopy study
Experimental subarachnoid hemorrhage causes early and long-lasting microarterial constriction and microthrombosis: an in-vivo microscopy study
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DOI：
10.1038/jcbfm.2011.154
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发表时间：
2012-03-01
影响因子：
6.3
通讯作者：
Plesnila, Nikolaus
中科院分区：
文献类型：
--
作者：
Friedrich, Benjamin;Mueller, Frank;Plesnila, Nikolaus
蛛网膜下腔出血（SAH）后早期脑损伤（EBI）的特征是严重的脑灌注压（CPP）非依赖性脑血流量减少，提示脑微血管水平的改变。因此，我们的目的是使用在体成像研究实验性SAH后的脑微循环。通过血管内穿孔诱导C57/BL 6小鼠蛛网膜下腔出血。在SAH后3、6和72小时，使用体内荧光显微镜检查软脑膜小动脉和小静脉（直径10至80 μ m）。小静脉直径或流量不受SAH的影响，而出血后3天，>70%的小动脉收缩22%至33%（与假手术相比，P < 0.05）。小动脉直径越小，收缩越明显（r（2）= 0.92，P
Early brain injury (EBI) after subarachnoid hemorrhage (SAH) is characterized by a severe, cerebral perfusion pressure (CPP)-independent reduction in cerebral blood flow suggesting alterations on the level of cerebral microvessels. Therefore, we aimed to use in-vivo imaging to investigate the cerebral microcirculation after experimental SAH. Subarachnoid hemorrhage was induced in C57/BL6 mice by endovascular perforation. Pial arterioles and venules (10 to 80 mu m diameter) were examined using in-vivo fluorescence microscopy, 3, 6, and 72 hours after SAH. Venular diameter or flow was not affected by SAH, while >70% of arterioles constricted by 22% to 33% up to 3 days after hemorrhage (P < 0.05 versus sham). The smaller the investigated arterioles, the more pronounced the constriction (r(2) = 0.92, P