Stress-induced formation of cell wall-deficient cells in filamentous actinomycetes
Stress-induced formation of cell wall-deficient cells in filamentous actinomycetes
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DOI：
10.1038/s41467-018-07560-9
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发表时间：
2018-12-04
影响因子：
16.6
通讯作者：
Claessen, Dennis
中科院分区：
文献类型：
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作者：
Ramijan, Karina;Ultee, Eveline;Claessen, Dennis
细胞壁是一种形状限定结构，几乎包裹所有细菌，并保护它们免受环境压力的影响。在某些干扰细胞壁合成的条件下，细菌可以被迫在没有细胞壁的情况下生长，但这些无壁细胞（称为L型）的相关性尚不清楚。在这里，我们表明，几种丝状放线菌有一种天然的能力，产生壁缺陷的细胞在高渗透胁迫下，我们称之为S细胞。这种壁缺陷状态是短暂的，因为S细胞能够切换到正常的菌丝生长模式。然而，S细胞长期暴露于高渗应激产生的变异体能够无限期地增殖，没有他们的细胞壁，类似于L型。我们认为放线菌细胞壁缺陷的形成可能是对渗透胁迫的一种适应。
The cell wall is a shape-defining structure that envelopes almost all bacteria and protects them from environmental stresses. Bacteria can be forced to grow without a cell wall under certain conditions that interfere with cell wall synthesis, but the relevance of these wall-less cells (known as L-forms) is unclear. Here, we show that several species of filamentous actinomycetes have a natural ability to generate wall-deficient cells in response to hyper osmotic stress, which we call S-cells. This wall-deficient state is transient, as S-cells are able to switch to the normal mycelial mode of growth. However, prolonged exposure of S-cells to hyperosmotic stress yields variants that are able to proliferate indefinitely without their cell wall, similarly to L-forms. We propose that formation of wall-deficient cells in actinomycetes may serve as an adaptation to osmotic stress.