GENETIC-CONTROL OF CELL-DIVISION PATTERNS IN THE DROSOPHILA EMBRYO
GENETIC-CONTROL OF CELL-DIVISION PATTERNS IN THE DROSOPHILA EMBRYO
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DOI：
10.1016/0092-8674(89)90183-9
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发表时间：
1989-04-07
期刊：
影响因子：
64.5
通讯作者：
OFARRELL, PH
中科院分区：
文献类型：
--
作者：
EDGAR, BA;OFARRELL, PH
在果蝇胚胎发育中，有丝分裂控制在第14个间期经历了一个显著的转变。14期之前的有丝分裂发生在母体产物上，并出现在准异步波中。间期14之后的有丝分裂需要合子转录，并以错综复杂、高度有序的时空模式异步发生。在这一转变过程中，在14号间期的G2中，字符串(Stg)基因的突变会导致细胞周期停滞，但不会阻止发育的其他方面。这种表型表明，stg是启动有丝分裂所必需的。我们描述了stg的克隆，并表明其预测的氨基酸序列与酵母裂殖酵母有丝分裂起始调节因子CDC25的氨基酸序列同源。此外，我们还发现，stg mRNA的合子表达发生在一系列动态的空间模式中，这些模式预测了合子驱动的细胞分裂的模式。因此，我们认为，stg mRNA的调节表达控制着这些胚胎细胞分裂的时间和位置。
In Drosophila embryogenesis, mitotic control undergoes a significant transition during the 14th interphase. Mitoses before interphase 14 run on maternal products, and occur in metasynchronous waves. Mitoses after interphase 14 require zygotic transcription, and occur asynchronously in an intricate, highly ordered spatio-temporal pattern. Mutations at the string (stg) locus cause cell-cycle arrest during this transition, in G2 of interphase 14, yet do not arrest other aspects of development. This phenotype suggests that stg is required specifically for initiating mitosis. We describe the cloning of stg, and show that its predicted amino acid sequence is homologous to that of cdc25, a regulator of mitotic initiation in the yeast Schizosaccharomyces pombe. In addition, we show that zygotic expression of stg mRNa occurs in a dynamic series of spatial patterns which anticipate the patterns of the zygotically driven cell divisions. Therefore we suggest that regulated expression of stg mRNA controls the timing and location of these embryonic cell divisions.