Preparation of biocompatible molecularly imprinted shell on superparamagnetic iron oxide nanoparticles for selective depletion of bovine hemoglobin in biological sample.
Preparation of biocompatible molecularly imprinted shell on superparamagnetic iron oxide nanoparticles for selective depletion of bovine hemoglobin in biological sample.
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DOI：
10.1016/j.jcis.2016.02.051
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发表时间：
2016-05
影响因子：
9.9
通讯作者：
Yi Hao;Ruixia Gao;Dechun Liu;Bianbian Zhang;Yuhai Tang;Zengjun Guo
中科院分区：
文献类型：
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作者：
Yi Hao;Ruixia Gao;Dechun Liu;Bianbian Zhang;Yuhai Tang;Zengjun Guo
牛血红蛋白（BHb）作为高丰度蛋白质之一，会严重掩盖和阻碍血清中低丰度蛋白质的分析。为了选择性地消耗BHb，我们设计了一种简单有效的策略，通过表面印迹技术结合模板固定化策略，在超顺磁性氧化铁纳米颗粒上制备生物相容性分子印迹壳。首先，模板蛋白通过亚胺键固定在直接醛功能化的磁性纳米颗粒上。然后，以明胶作为功能单体，获得围绕固定化模板蛋白模制的聚合物网络。最后，去除模板蛋白后，制造出 BHb 的特定空腔。研究了印迹条件的影响，发现最佳印迹条件为 40 mg BHb、150 mg 明胶和 8 小时聚合时间。所得材料表现出良好的分散性、高结晶度和令人满意的超顺磁性能以及高饱和磁化强度（33.43 emu g−1）。吸附实验表明，印迹纳米材料具有93.1 mg g−1的高吸附容量、35 min的快速平衡时间以及对目标蛋白良好的选择性。同时，所得聚合物经过6次吸附-解吸循环后仍可继续使用，无明显劣化。此外，所得聚合物成功应用于从牛血液样本中选择性分离BHb，为蛋白质组学的制备工作提供了替代解决方案。
Bovine hemoglobin (BHb), as one of the high-abundance proteins, could seriously mask and hamper the analysis of low-abundance proteins in serum. To selectively deplete BHb, we design a simple and effective strategy for preparation of biocompatible molecularly imprinted shell on superparamagnetic iron oxide nanoparticles through surface imprinting technique combined with template immobilization strategy. Firstly, template proteins are immobilized on the directly aldehyde-functionalized magnetic nanoparticles through imine bonds. Then, with gelatin as functional monomer, a polymeric network molded around the immobilized template proteins is obtained. Finally, the specific cavities for BHb are fabricated after removing the template proteins. The effects of imprinting conditions were investigated and the optimal imprinting conditions are found to be 40 mg of BHb, 150 mg of gelatin, and 8 h of polymerization time. The resultant materials exhibit good dispersion, high crystallinity, and satisfactory superparamagnetic property with a high saturation magnetization (33.43 emu g−1). The adsorption experiments show that the imprinted nanomaterials have high adsorption capacity of 93.1 mg g−1, fast equilibrium time of 35 min, and satisfactory selectivity for target protein. Meanwhile, the obtained polymers could be used without obvious deterioration after six adsorption-desorption cycles. In addition, the resultant polymers are successfully applied in the selective isolation BHb from bovine blood sample, which could provide an alternative solution for the preparatory work of proteomics.