Using a diode laser for laser-induced fluorescence
Using a diode laser for laser-induced fluorescence
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DOI：
10.1021/ed078p1093
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发表时间：
2001-08-01
影响因子：
3
通讯作者：
Whitten, JE
中科院分区：
文献类型：
--
作者：
Tran, Y;Whitten, JE
介绍了一种由635 nm红光二极管激光器和放大光电二极管检测器组成的激光荧光计的结构和使用。二极管激光器的低成本和单色性质使其作为教育荧光实验的激发源具有吸引力。通过测量溶解在甲醇中的各种浓度的尼罗蓝A的荧光信号来证明这种类型的荧光计的使用;低至1 ppb的浓度很容易检测到。通过测量加入次氯酸钠后1 ppm尼罗蓝A溶液的荧光衰减作为时间的函数，证明了使用该仪器监测氧化剂对染料的分解。
The construction and use of a laser fluorimeter from a 635-nm red diode laser and an amplified photodiode detector are described. The low cost and monochromatic nature of diode lasers make them attractive as excitation sources for educational fluorescence experiments. Use of this type of fluorimeter is demonstrated by measuring fluorescence signals for various concentrations of Nile blue A dissolved in methanol; concentrations as low as 1 ppb are easily detected. The use of this instrument for monitoring the decomposition of a dye by an oxidizing agent is demonstrated by measuring the decay of fluorescence as a function of time for a 1 ppm Nile blue A solution after the addition of sodium hypochlorite.