tailup, A LIM-HD gene, and Iro-C cooperate in Drosophila dorsal mesothorax specification
tailup, A LIM-HD gene, and Iro-C cooperate in Drosophila dorsal mesothorax specification
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DOI：
10.1242/dev.02844
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发表时间：
2007-05-01
期刊：
影响因子：
4.6
通讯作者：
Modolell, Juan
中科院分区：
文献类型：
--
作者：
de Navascues, Joaquin;Modolell, Juan
LIM-HD基因tailup（也称为islet）已被归类为前模式基因，其通过下调前神经无毛鳞片基因的表达来拮抗果蝇背上感觉刚毛的形成。在这里，我们表明，在成虫翅盘的发展，即，指定的背领域，有一个较早的功能。缺乏翼细胞功能会导致这种原基的细胞上调不同的翼铰链基因，并失去一些背基因的表达。同时，这些细胞分化铰链结构或修改背角质层。LIM-HD辅因子Chip和SSDP对于notum规范也是必要的。这表明Tup与Chip和Ssdp在此过程中起作用。过表达的araucan，与一个'pronotum'基因的易洛魁族复杂的（Iro-C），协同加强弱能力的基因，单独过表达时，诱导异位notum样的发展。尽管Iro-C基因在背原基中通过EGFR信号传导被激活，但Iro-C基因通过Dpp信号传导被正调控。我们的数据支持一个模型，其中EGFR和Dpp信号通路，与它们各自的下游Iro-C和EkB基因，收敛和合作，使细胞的背发育的命运。
The LIM-HD gene tailup (tup; also known as islet) has been categorised as a prepattern gene that antagonises the formation of sensory bristles on the notum of Drosophila by downregulating the expression of the proneural achaete-scute genes. Here we show that tup has an earlier function in the development of the imaginal wing disc; namely, the specification of the notum territory. Absence of tup function causes cells of this anlage to upregulate different wing-hinge genes and to lose expression of some notum genes. Consistently, these cells differentiate hinge structures or modified notum cuticle. The LIM-HD co- factors Chip and Ssdp are also necessary for notum specification. This suggests that Tup acts in this process in a complex with Chip and Ssdp. Overexpression of tup, together with araucan, a 'pronotum' gene of the iroquois complex (Iro-C), synergistically reinforces the weak capacity of either gene, when overexpressed singly, to induce ectopic notum-like development. Whereas the Iro-C genes are activated in the notum anlage by EGFR signalling, tup is positively regulated by Dpp signalling. Our data support a model in which the EGFR and Dpp signalling pathways, with their respective downstream Iro-C and tup genes, converge and cooperate to commit cells to the notum developmental fate.