Thermophilic archaeal community succession and function change associated with the leaching rate in bioleaching of chalcopyrite.
Thermophilic archaeal community succession and function change associated with the leaching rate in bioleaching of chalcopyrite.
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DOI：
10.1016/j.biortech.2013.01.135
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发表时间：
2013-04
影响因子：
11.4
通讯作者：
Weixiong Zhu;J. Xia;Yi Yang;Z. Nie;An-an Peng;Hongchang Liu;G. Qiu
中科院分区：
文献类型：
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作者：
Weixiong Zhu;J. Xia;Yi Yang;Z. Nie;An-an Peng;Hongchang Liu;G. Qiu
采用变性梯度凝胶电泳（DGGE）分析16S rRNA基因扩增片段，实时qPCR分析与黄铜矿生物浸出率相关的硫氧化soxB基因扩增片段，研究了嗜热古细菌brierleyi、Metallosphaera seula、Acidianus manzaensis和Sulfolobus metallicus的群落演替和功能变化。群落演替分析结果表明，温度和初始pH值对群落演替有显著影响，其中S. metallicus对环境变化最敏感，a . brierleyi对环境的适应性和硫氧化能力最好，在各浸出体系中均占主导地位。同时，黄铜矿的浸出速率与soxB基因所体现的联合体功能密切相关，为揭示微生物硫氧化差异和追踪联合体功能变化，优化浸出参数，提高有价金属的回收率提供了良好的途径。
The community succession and function change of thermophilic archaea Acidianus brierleyi, Metallosphaera sedula, Acidianus manzaensis and Sulfolobus metallicus were studied by denaturing gradient gel electrophoresis (DGGE) analysis of amplifying 16S rRNA genes fragments and real-time qPCR analysis of amplifying sulfur-oxidizing soxB gene associated with chalcopyrite bioleaching rate at different temperatures and initial pH values. The analysis results of the community succession indicated that temperature and initial pH value had a significant effect on the consortium, and S. metallicus was most sensitive to the environmental change, A. brierleyi showed the best adaptability and sulfur oxidation ability and predominated in various leaching systems. Meanwhile, the leaching rate of chalcopyrite closely related to the consortium function embodied by soxB gene, which could prove a desirable way for revealing microbial sulfur oxidation difference and tracking the function change of the consortium, and for optimizing the leaching parameters and improving the recovery of valuable metals.