Amniotic membrane as support for human retinal pigment epithelium (RPE) cell growth
Amniotic membrane as support for human retinal pigment epithelium (RPE) cell growth
复制标题
DOI：
10.1034/j.1600-0420.2003.00076.x
复制
发表时间：
2003-06-01
期刊：
影响因子：
--
通讯作者：
Sánchez-Salorio, M
中科院分区：
文献类型：
--
作者：
Capeáns, C;Piñeiro, A;Sánchez-Salorio, M
目的：这项工作的目的是在人羊膜 (hAM) 上培养人视网膜色素上皮 (hRPE) 细胞。研究了人 AM 作为将保留细胞极性的 hRPE 单层细胞移植到视网膜下腔的充分支持的可行性。方法：人 AM 是从剖腹产期间的孕妇中获得的。将 hAM 切片并将碎片固定在培养皿上。人类 RPE 细胞是从成年角膜供体中培养出来的，并接种在 hAM 上。获得相差照片。选定的标本通过透射电子显微镜（TEM）进行处理。结果：观察到hRPE细胞在hAM上的附着和生长。人类 RPE 细胞构成紧密的集落，维持上皮表型。使用 TEM，我们鉴定了单层 hRPE 细胞，具有立方体到球形的形态。这些细胞显示出与基质的整合，并且检测到细胞与细胞的接触。结论：羊膜可能是hRPE生长的合适基质。需要进一步研究以确定 hRPE 对视网膜下腔 hAM 的活力。
Purpose: The aim of this work was to culture human retinal pigment epithelium (hRPE) cells over human amniotic membrane (hAM). Human AM was studied for its viability as an adequate support for transplantation of an hRPE cell monolayer with preserved cell polarity to the subretinal space.Methods: Human AM was obtained from pregnant women during caesarean section. The hAM was sectioned and the pieces were fixed to culture dishes. Human RPE cells were cultured from adult corneal donors and were seeded over hAM. Phase-contrast photographs were obtained. Selected specimens were processed by transmission electronic microscopy (TEM).Results: The attachment and growth of hRPE cells over hAM was observed. Human RPE cells constituted tight colonies that maintained epithelial phenotype. Using TEM, we identified a monolayer of hRPE cells, with cuboidal to spheroidal morphology. These cells showed integration with the substrate and cell-cell contacts were detected.Conclusion: Amniotic membrane may be a suitable substrate for hRPE growth. Further studies are required in order to determine the viability of hRPE on hAM in the subretinal space.