Amphioxus ribosomal proteins RPS15, RPS18, RPS19 and RPS30-precursor act as immune effectors via killing or agglutinating bacteria
Amphioxus ribosomal proteins RPS15, RPS18, RPS19 and RPS30-precursor act as immune effectors via killing or agglutinating bacteria
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DOI：
10.1016/j.fsi.2021.09.001
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发表时间：
2021-09-06
影响因子：
4.7
通讯作者：
Gao, Zhan
中科院分区：
文献类型：
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作者：
Chen, Chaoyi;Yuan, Jianrui;Gao, Zhan
以往的研究表明，一些核糖体蛋白通过直接杀死细菌来实现免疫效应功能。然而，在细菌感染过程中，核糖体蛋白的其他效应功能在很大程度上仍然未知。本研究表达并纯化了文昌鱼（Branchiostoma japonicum）核糖体蛋白rBjRPS 15、rBjRPS 18、rBjRPS 19和rBjRPS 30-precursor（rBjRPS 30 P）。除rBjRPS 19和rBjRPS 30 P外，其余菌株均对革兰氏阳性金黄色葡萄球菌具有杀菌活性，对革兰氏阴性大肠杆菌具有杀灭作用。rBjRPS 15、rBjRPS 19和rBjRPS 30 P能凝集S.金黄色葡萄球菌（E. aureus）在Mg ~（2+）存在下凝集，但均不能凝集E.即使在Mg ~（2+）或Ca ~（2+）存在下，此外，S.金黄色葡萄球菌凝集是通过这三种蛋白质与细菌细胞壁的肽聚糖组分结合而实现的。这是首次报道某些核糖体蛋白具有细菌凝集活性，这些数据为核糖体蛋白在免疫防御中的作用提供了一个新的角度。
Previous studies show that some ribosomal proteins perform immune effector functions via killing bacteria directly. However, it remains largely unknown about other effector functions of ribosomal proteins during a bacterial infection. In this study, we expressed and purified four ribosomal proteins of the amphioxus Branchiostoma japonicum, termed rBjRPS15, rBjRPS18, rBjRPS19 and rBjRPS30-precursor (rBjRPS30P). They all exhibited bactericidal activity against Gram-positive Staphylococcus aureus, and with the exception of rBjRPS19 and rBjRPS30P, were capable of killing Gram-negative Escherichia coli. Importantly, rBjRPS15, rBjRPS19 and rBjRPS30P were able to agglutinate S. aureus in the presence of Mg2+, but none of them could agglutinate E. coli even in the presence of Mg2+ or Ca2+. Moreover, the S. aureus agglutination was achieved by the binding of these three proteins to the peptidoglycan component of the bacterial cell wall. This is the first report showing that some ribosomal proteins possess bacterial agglutinating activity, and these data provide a new angle to the roles of ribosomal proteins in immune defense.