Expression of lectin-like oxidized LDL receptor-1 in smooth muscle cells after vascular injury
Expression of lectin-like oxidized LDL receptor-1 in smooth muscle cells after vascular injury
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DOI：
10.1016/j.bbrc.2005.12.211
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发表时间：
2006-03-10
影响因子：
3.1
通讯作者：
Tei, C
中科院分区：
文献类型：
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作者：
Eto, H;Miyata, M;Tei, C
凝集素样氧化低密度脂蛋白受体-1（LOX-1）是一种氧化型低密度脂蛋白受体，其在血管成形术后再狭窄中的作用尚不清楚。我们采用兔主动脉球囊损伤模型，逆转录-聚合酶链反应显示，LOX-1 mRNA的表达在未损伤的主动脉中是适度的，在损伤后2天达到峰值水平，并且在损伤后24周一直保持升高。免疫组化和原位杂交结果显示，损伤后2周和24周，LOX-1在正常主动脉中膜中无表达，但在中膜和新生内膜平滑肌细胞（SMC）中均有表达。低浓度ox-LDL（10 μ g/mL）刺激SMC增殖，LOX-1 mRNA反义寡核苷酸抑制SMC增殖。双重免疫荧光染色显示，LOX-1和增殖细胞核抗原在人类再狭窄病变中共存。这些结果表明LOX-1介导ox-LDL诱导的SMC增殖，并在血管损伤后新生内膜形成中发挥作用。(c)2006年爱思唯尔公司All rights reserved.
Lectin-like oxidized LDL receptor-1 (LOX-1) is an oxidized LDL receptor, and its role in restenosis after angioplasty remains unknown. We used a balloon-injury model of rabbit aorta, and reverse transcription-polymerase chain reaction revealed that LOX-1 mRNA expression was modest in the non-injured aorta, reached a peak level 2 days after injury, and remained elevated Until 24 weeks after injury. Immunohistochemistry and in situ hybridization showed that LOX-1 was not detected in the media of non-injured aorta but expressed in both medial and neointimal Smooth muscle cells (SMC) at 2 and 24 weeks after injury. Low concentrations of ox-LDL (10 mu g/mL) stimulated the cultured SMC proliferation, which was inhibited by antisense oligonucleotides of LOX-1 mRNA. Double immunofluorescense staining showed the colocalization of LOX-1 and proliferating cell nuclear antigen in human restenotic lesion. These results suggest that LOX-1 mediates ox-LDL-induced SMC proliferation and plays a role in neointimal formation after vascular injury. (c) 2006 Elsevier Inc. All rights reserved.