Proliferation and differentiation of cultured MC3T3-E1 osteoblasts on surface-layer modified hydroxyapatite ceramic with acid and heat treatments.
Proliferation and differentiation of cultured MC3T3-E1 osteoblasts on surface-layer modified hydroxyapatite ceramic with acid and heat treatments.
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DOI：
10.4012/dmj.24.207
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发表时间：
2005
影响因子：
2.5
通讯作者：
T. Yuasa;Y. Miyamoto;M. Kon;K. Ishikawa;M. Takechi;Y. Momota;S. Tatehara;H. Takano;Shiho Mimamiguchi;M. Nagayama
中科院分区：
文献类型：
--
作者：
T. Yuasa;Y. Miyamoto;M. Kon;K. Ishikawa;M. Takechi;Y. Momota;S. Tatehara;H. Takano;Shiho Mimamiguchi;M. Nagayama
使用MC3T3-E1细胞评估由羟基磷灰石（HAP）和α-磷酸三钙（α-TCP）组成的功能梯度磷酸钙对成骨细胞增殖和分化的影响。 HAP-α-TCP功能梯度磷酸钙、纯HAP和细胞培养塑料孔之间MC3T3-E1细胞的增殖没有显着差异。以I型胶原、碱性磷酸盐、骨钙素的mRNA表达作为初始评价指标；成骨细胞分化的中期和晚期。基本上，与细胞培养塑料孔相比，HAP-α-TCP 功能梯度磷酸钙和纯 HAP 增强了三种标记物的表达。对于 I 型胶原蛋白和碱性磷酸盐的表达，HAP-α-TCP 功能梯度磷酸钙显示出与纯 HAP 相同的表达水平。对于骨钙素表达，HAP-α-TCP 功能梯度磷酸钙显示出比纯 HAP 更高的水平。因此，我们得出结论，HAP-α-TCP功能梯度磷酸钙具有作为高骨传导性骨填充材料的良好潜力。
Effects of functionally gradient calcium phosphate consisting of hydroxyapatite (HAP) and alpha-tricalcium phosphate (alpha-TCP) on proliferation and differentiation of osteoblasts were evaluated using MC3T3-E1 cells. There were no significant differences in the proliferation of MC3T3-E1 cells among HAP-alpha-TCP functionally gradient calcium phosphate, pure HAP, and cell culture plastic wells. mRNA expressions of type I collagen, alkaline phosphate, and osteocalcine were evaluated as indexes of initial; mid-stage, and late-stage osteoblastic differentiation. Basically, HAP-alpha-TCP functionally gradient calcium phosphate and pure HAP enhanced the expressions of the three markers when compared with that of cell culture plastic wells. For type I collagen and alkaline phosphate expressions, HAP-alpha-TCP functionally gradient calcium phosphate showed the same expression level as pure HAP. For osteocalcine expression, HAP-alpha-TCP functionally gradient calcium phosphate showed a higher level than pure HAP. We concluded, therefore, HAP-alpha-TCP functionally gradient calcium phosphate has good potential to be a bone filler material with high osteoconductivity.