Comparison of stress-induced modulation of smooth-muscle activity between ileum and colon in male rats
Comparison of stress-induced modulation of smooth-muscle activity between ileum and colon in male rats
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DOI：
10.1016/j.autneu.2014.01.008
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发表时间：
2014-07-01
影响因子：
2.7
通讯作者：
Toda, Kazuo
中科院分区：
文献类型：
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作者：
Kimoto, Mari;Zeredo, Jorge L.;Toda, Kazuo
众所周知，压力是许多消化系统疾病的原因，包括胃肠道功能紊乱。自主神经系统通过胆碱能和肾上腺素能传出纤维调节肠道运动；然而，目前尚不清楚压力如何影响这些控制机制，特别是是否以地点依赖的方式。在体外实验中，我们检测了乙酰胆碱（Ach）和肾上腺素（Adr）对应激大鼠肠段平滑肌收缩的影响。通过超重力刺激（10分钟/天）加载压力，持续1、6或30天。因此，应激条件对回肠和结肠部分肠道对乙酰胆碱和Adr的敏感性有不同的影响。在回肠中，对照组和应激条件大鼠之间没有发现显著差异，而在结肠中，6天和30天应激条件大鼠的样本显示出更大的疼痛诱导的收缩幅度，以及更大的Adr应用拮抗作用。这些结果表明，应激条件可以通过改变平滑肌对乙酰胆碱和Adr的敏感性来改变肠道运动的自主控制。(C) 2014 Elsevier B.V.版权所有
Stress is a well-known cause of numerous digestive conditions, including gastrointestinal-function disorders. The autonomic nervous system regulates intestinal movements via cholinergic and adrenergic efferent fibers; however it is not clear how stress could affect these control mechanisms and in particular whether in a site-dependent manner. In this study we tested in vitro the effects of topical application of acetylcholine (Ach) and adrenalin (Adr) on smooth-muscle contractions of intestinal segments isolated from stress-conditioned rats. Stress was loaded by hypergravity stimulation (10 min/day) for periods of 1, 6 or 30 days. As a result, stress-conditioning affected intestinal sensitivity to Ach and Adr differently at sections of the ileum and colon. In the ileum no significant differences were found between control and stress-conditioned rats, whereas in the colon, samples from 6- and 30-day stress-conditioned rats showed larger amplitudes of Ach-induced contraction, as well as greater antagonization by Adr application. These results suggest that stress conditioning can modify autonomic control of intestinal movements by altering smooth-muscle sensitivity to Ach and Adr. (C) 2014 Elsevier B.V. All rights reserved.