Photosynthetic oxygen evolution in mesoporous silica material: adsorption of photosystem II reaction center complex into 23 nm nanopores in SBA.
Photosynthetic oxygen evolution in mesoporous silica material: adsorption of photosystem II reaction center complex into 23 nm nanopores in SBA.
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DOI：
10.1021/la1032916
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发表时间：
2011-01
期刊：
影响因子：
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通讯作者：
Tomoyasu Noji;Chihiro Kamidaki;K. Kawakami;Jian-Ren Shen;T. Kajino;Y. Fukushima;T. Sekitoh;S. Itoh
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文献类型：
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作者：
Tomoyasu Noji;Chihiro Kamidaki;K. Kawakami;Jian-Ren Shen;T. Kajino;Y. Fukushima;T. Sekitoh;S. Itoh
放氧光合反应中心复合物（PSII）被吸附到SBA，介孔二氧化硅化合物的纳米孔。我们从嗜热蓝细菌Thermosynechococcus vulcanus中纯化了PSII复合物的二聚体，其最佳生长温度为57 °C。热稳定的PSII二聚体复合物具有20 nm的直径和756 kDa的分子量，并结合超过60个叶绿素。平均内孔径为15 nm（SBA（15））和23 nm（SBA（23））的SBA颗粒分别吸附4.7和15 mg PSII/g SBA。用共聚焦激光扫描显微镜的测量表明PSII吸附到SBA（23）颗粒的表面和内部空间，表明PSII吸附到23 nm二氧化硅纳米孔中。PSII不与SBA的内孔结合（15）。结合到SBA（23）的PSII显示出光合放氧反应的高且稳定的活性，表明光驱动的电子从水传递到外部介质中添加的醌分子。PSII-SBA偶联物有望成为光传感器和人工光合系统的新材料。
An oxygen-evolving photosynthetic reaction center complex (PSII) was adsorbed into nanopores in SBA, a mesoporous silica compound. We purified the dimer of PSII complex from a thermophilic cyanobacterium, Thermosynechococcus vulcanus, which grows optimally at 57 °C. The thermally stable PSII dimeric complex has a diameter of 20 nm and a molecular mass of 756 kDa and binds more than 60 chlorophylls. The SBA particles, with average internal pore diameters of 15 nm (SBA(15)) and 23 nm (SBA(23)), adsorbed 4.7 and 15 mg of PSII/g SBA, respectively. Measurement with a confocal laser-scanning microscope indicated the adsorption of PSII to the surface and the inner space of the SBA(23) particles, indicating the adsorption of PSII into the 23 nm silica nanopores. PSII did not bind to the inner pores of SBA(15). PSII bound to SBA(23) showed the high and stable activity of a photosynthetic oxygen-evolving reaction, indicating the light-driven electron transport from water to the quinone molecules added in the outer medium. The PSII-SBA conjugate can be a new material for photosensors and artificial photosynthetic systems.