Increased cyclin E expression may obviate the role of cyclin D1 during brain development in cyclin D1 knockout mice
Increased cyclin E expression may obviate the role of cyclin D1 during brain development in cyclin D1 knockout mice
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DOI：
10.1111/j.1471-4159.2004.02934.x
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发表时间：
2005-03-01
影响因子：
4.7
通讯作者：
Zhu, J
中科院分区：
文献类型：
--
作者：
Chen, Z;Duan, RS;Zhu, J
细胞周期蛋白D和E在哺乳动物细胞分裂的G1期起关键作用。细胞周期蛋白D1高表达，有望在小鼠脑发育过程中发挥重要作用。然而，在本研究中，我们在1月龄、4月龄和12月龄时发现Cyclin D1基因敲除小鼠(KO)和对照野生型小鼠的中枢神经系统形态没有差异。对胎脑蛋白质表达的分析表明，在出生后13.5天，细胞周期蛋白E在细胞周期蛋白D1KO小鼠体内的表达明显增加。在同一年龄段的野生型小鼠胚胎脑中检测到高水平的细胞周期蛋白D1的表达。提示Cyclin D1KO小鼠脑内Cyclin E蛋白表达增强，可能不再发挥Cyclin D1KO小鼠的作用，有助于其正常脑发育。
Cyclins D and E play critical roles during the G1 phase of mammalian cell division. Cyclin D1 expression is high and expected to play an important role during mouse brain development. However, in the present study, we found no difference in CNS morphology between cyclin D1 knockout (KO) and control wild-type mice at the ages of 1, 4 and 12 months. Analysis of protein expression in embryonic brains revealed that cyclin E is obviously increased in cyclin D1 KO mice at 13.5 days post coitum. At the same age a high level of cyclin D1 expression is detected in the embryonic brain of wild-type mice. The data indicate that enhanced cyclin E protein expression in cyclin D1 KO mice may obviate the role of cyclin D1 and contribute to the normal brain development of cyclin D1 KO mice.