Investigation of collisional-activation decomposition process and spectra in the transport region of an electrospray single-quadrupole mass spectrometer.
Investigation of collisional-activation decomposition process and spectra in the transport region of an electrospray single-quadrupole mass spectrometer.
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研究电喷雾单四极杆质谱仪输运区的碰撞活化分解过程和光谱。
DOI：
10.1002/rcm.1290050604
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Pack,T
中科院分区：
文献类型：
--
作者：
Voyksner,RD;Pack,T
评价了在电喷雾区域中使用碰撞活化解离（CAD）以生成关于几种农药和抗生素的结构信息。用于生成CAD谱的碰撞能量可以通过改变毛细管/撇渣器电势差来容易地改变，从而将从OeV到16 eV以上的内能赋予通过电喷雾生成的近热离子。低能碰撞的内能分布（毛细管/撇渣器电势差为20 V）与由三重四极质谱仪生成的曲线紧密匹配。此外，在30-50 V的毛细管/撇渣器电位差下，通过电喷雾在传输区产生的所选化合物的CAD光谱与通过使用30 eV碰撞能量的三重四极杆从[M + H]+离子获得的那些非常相似。与质子化或阳离子化母体分子的离子电流相比，运输区域中离子的CAD导致70%至80%的子离子产率和总离子电流的最小损失。在全扫描CAD条件下，可检测到10 pg涕灭威和青霉素G的主要子离子（信噪比>5）。
The use of collisional‐activation dissociation (CAD) in the electrospray region was evaluated for generating structural information on several pesticides and antibiotics. The collision energy used to generate the CAD spectra could be varied easily by changing the capillary/skimmer potential difference, imparting fromOeV to above 16 eV internal energy to the near thermal ions generated by electrospray, The internal energy distribution for low‐energy collisions (capillary/skimmer potential difference of 20 V) closely matches the curves generated by a triple‐quadrupole mass spectrometer. Furthermore, the CAD spectra for selected compounds generated by electrospray in the transport region at a capillary/skimmer potential differences of 30–50 V closly resembled those obtained from the [M + H]+ion by a triple quadrupole using 30eV collision energy. The CAD of ions in the transport region resulted in 70% to 80% daughter‐ion yields and minimal loss in overall ion current compared to the ion current for protonated or cationized parent molecules. The major daughter ions for 10 pg of Aldicarb and penicillin G could be detected (signal‐to‐noise ratio>5) under full‐scan CAD conditions.