Endothelin-1 chronically inhibits Na/H exchanger-3 in ETB-overexpressing OKP cells
Endothelin-1 chronically inhibits Na/H exchanger-3 in ETB-overexpressing OKP cells
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DOI：
10.1006/bbrc.2000.2724
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发表时间：
2000-05-19
影响因子：
3.1
通讯作者：
Hsieh, BS
中科院分区：
文献类型：
--
作者：
Chu, TS;Wu, KD;Hsieh, BS
内皮素-1(ET-1)可显著增强负鼠肾小管上皮细胞系OKPET(B)6细胞的Na/H逆向转运蛋白活性。本研究的目的是观察ET-1对OKP细胞Na/H逆向转运蛋白活性的慢性影响及其对Na/H交换器(NHE)-3mRNA和蛋白丰度的调节作用，用10 nM ET-1处理静止的OKPET(B)6细胞3、6和24 h，检测Na/H逆向转运蛋白活性。3-h ET-1处理的细胞Na/H逆向转运蛋白活性与对照组无明显差异。ETB受体拮抗剂BQ788可阻断ET-1对Na/H逆向转运蛋白活性的影响，而ETA受体拮抗剂BQ123不能阻断ET-1对Na/H逆向转运蛋白活性的影响。ET-1处理细胞3h时NHE-3mRNA丰度与对照组无明显差异，但在6h和24h NHE-3mRNA丰度显著降低，NHE-3蛋白丰度在6h和24h显著降低。(C)2000年学术出版社。
Endothelin-1 (ET-1) acutely increases Na/H antiporter activity in OKPET(B)6 cells, an opossum kidney proximal tubule cell line transfected with ETB receptor cDNA. The purpose of the present study was to examine the chronic effect of ET-1 on Na/H antiporter activity in OKP cells and to examine whether Na/H exchanger (NHE)-3 mRNA and protein abundance are regulated by ET-1, Quiescent OKPET(B)6 cells were treated with 10 nM ET-1 for 3, 6 or 24 h and Na/H antiporter activity was assayed. The Na/H antiporter activity in 3-h ET-1-treated cells was not different from controls. However, Na/H antiporter activity was significantly decreased by 29% at 6 h and 72% at 24 h. The effect of ET-1 on Na/H antiporter activity was blocked by BQ788, an ETB receptor antagonist, but not BQ123, an ETA receptor antagonist, The NHE-3 mRNA abundance in ET-1-treated cells was not different from controls at 3 h, However, there was a significant decrease in NHE-3 mRNA abundance at 6 and 24 h, There was also a significant decrease in NHE-3 protein abundance at 6 and 24 h. In summary, ET-1 chronically inhibits NHE-3 in OKPET(B)6 cells. (C) 2000 Academic Press.