Overexpression of miR-145-5p Inhibits Proliferation of Prostate Cancer Cells and Reduces SOX2 Expression
Overexpression of miR-145-5p Inhibits Proliferation of Prostate Cancer Cells and Reduces SOX2 Expression
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DOI：
10.3109/07357907.2015.1025407
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发表时间：
2015-01-01
影响因子：
2.4
通讯作者：
Ittmann, Michael
中科院分区：
文献类型：
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作者：
Ozen, Mustafa;Karatas, Omer Faruk;Ittmann, Michael
我们的目的是在前列腺癌（PCa）细胞中进行miR-145- 5 p的功能分析，并确定miR-145- 5 p的靶点，以了解其在PCa发病机制中的作用。PC 3、DU 145、LNCaP PCa和PNT 1a非致瘤前列腺细胞系用于miR-145- 5 p的功能分析。它的过度表达通过细胞凋亡和减少PCa细胞的迁移引起增殖抑制。在miR-145- 5 p过表达的PCa细胞中，SOX 2的表达在mRNA和蛋白水平上均显著降低。我们认为miR-145- 5 p是SOX 2的重要调节因子，在PCa肿瘤发生中起着至关重要的作用。
We aimed to perform functional analysis of miR-145-5p in prostate cancer (PCa) cells and to identify targets of miR-145-5p for understanding its role in PCa pathogenesis. PC3, DU145, LNCaP PCa, and PNT1a nontumorigenic prostate cell lines were utilized for functional analysis of miR-145-5p. Its overexpression caused inhibition of proliferation through apoptosis and reduced migration in PCa cells. SOX2 expression was significantly decreased in both mRNA and protein level in miR-145-5p-overexpressed PCa cells. We proposed that miR-145-5p, being an important regulator of SOX2, carries a crucial role in PCa tumorigenesis.