Responses of the solitary olfactory receptor cell of the guinea pig.
Responses of the solitary olfactory receptor cell of the guinea pig.
复制标题
豚鼠孤立嗅觉受体细胞的反应。
DOI：
10.3109/00016489209137465
复制
发表时间：
1992
影响因子：
1.4
通讯作者：
T. Kumazawa
中科院分区：
文献类型：
--
作者：
T. Inoue;T. Yamashita;N. Harada;T. Kumazawa
采用酶消化和机械分离的方法，从豚鼠鼻粘膜中分离出嗅感受器细胞。在相差显微镜下，由于形态特征，细胞可以容易地与其他组织区分开来。用钙敏感染料Fura-2和数字成像显微镜测定细胞内游离钙离子浓度（[Ca 2 +]i）。在1 μ M离子载体离子霉素存在下，所有细胞的[Ca ~（2+）]i都不可逆地增加。在标准条件下用10 mM乙酸正戊酯长时间刺激期间，40个分离的嗅细胞中有16个对[Ca 2 +]i有反应。有没有任何证据表明，在嗅觉受体细胞中的[Ca 2 +]i的增加在名义上无Ca 2+的溶液和刺激的乙酸戊酯。当加入3 mM CaCl_2时，[Ca ~（2+）]i显著增加。当细胞在补充有10 μ M维拉帕米（一种L型电压依赖性钙通道拮抗剂）的CaCl 2耗尽的细胞外液中用乙酸戊酯刺激时，[Ca 2 +]i无变化。向该液体中添加CaCl 2并没有升高任何细胞中的[Ca 2 +]i。这将表明，在乙酸戊酯的存在下，[Ca 2 +]i的增加可能主要取决于从细胞外空间的钙流入。我们的发现，细胞外钙流入细胞被维拉帕米抑制表明，钙通道，这是开放的乙酸戊酯，是一个电压依赖性的L型钙通道。
Olfactory receptor cells were isolated from the nasal mucosa of the guinea pig, using enzymatic digestion and mechanical dissociation. Under a phase-contrast microscope, the cells can be readily distinguished from other tissues, because of morphological characteristics. The intracellular free calcium ion concentrations ([Ca2+]i) of these cells were determined using the Ca2+ sensitive dye fura-2 and digital imaging microscopy. In the presence of 1 microM ionomycin, calcium-ionophore, there was an irreversible increase in [Ca2+]i in all the cells. During prolonged stimulation by 10 mM n-Amyl acetate under standard conditions, 16 of the 40 isolated olfactory cells responded with [Ca2+]i. There was no evidence of any increase in [Ca2+]i in the olfactory receptor cells in nominally Ca2+ free solution and stimulated by amyl acetate. When 3 mM CaCl2 was added, the [Ca2+]i increased remarkably. When cells were stimulated with amyl acetate in a CaCl2-depleted extracellular fluid supplemented with 10 microM verapamil (an L-type voltage-dependent calcium channel antagonist), no change occurred in [Ca2+]i. The addition of CaCl2 to this fluid did not elevate [Ca2+]i, in any cell. This would suggest that the [Ca2+]i increase in the presence of amyl acetate may mainly depend on the influx of calcium from the extracellular space. Our finding that the influx of extracellular calcium into cells was suppressed by verapamil suggests that the calcium channel, which is opened by amyl acetate, is a voltage-dependent L-type Ca2+ channel.
嗅觉神经元在去极化诱导的钙变化中表现出异质性。
DOI：
10.1152/ajpcell.1990.258.6.c1051
发表时间：
1990
期刊：
The American journal of physiology
影响因子：
--
作者：
Restrepo,D;Teeter,JH
通讯作者：
Teeter,JH