Helium ion microscopy of Lepidoptera scales
Helium ion microscopy of Lepidoptera scales
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DOI：
10.1002/sca.20267
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发表时间：
2012-03-01
期刊：
影响因子：
--
通讯作者：
Bagnall, Darren M.
中科院分区：
文献类型：
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作者：
Boden, Stuart A.;Asadollahbaik, Asa;Bagnall, Darren M.
在本报告中，使用氦离子显微镜 (HIM) 研究了凤蝶科两种鳞翅目蝴蝶翅膀结构颜色的微米和纳米结构：Papilio ulysses（蓝山蝴蝶）和 Parides sesostris（翡翠斑纹牛心蝶）。由于入射离子束，这些蝴蝶翅膀上未涂层鳞片的电子电荷被成功中和，从而导致图像显示出大景深和高水平的表面细节，而这些细节通常会被用于扫描电子显微镜 (SEM) 的传统涂层方法所掩盖。将这些图像与变压 SEM 的图像进行比较，证明了 HIM 在高放大倍数下的优越性。此外，通过创建立体对来利用 HIM 的大景深功能，从而允许探索三维。此外，还证明了定量高度信息的提取与文献中的横截面透射电子显微镜测量结果非常匹配。扫描 33: 107120, 2012。(c) 2011 Wiley periodicals, Inc.
In this report, helium ion microscopy (HIM) is used to study the micro and nanostructures responsible for structural color in the wings of two species of Lepidotera from the Papilionidae family: Papilio ulysses (Blue Mountain Butterfly) and Parides sesostris (Emerald-patched Cattleheart). Electronic charging of uncoated scales from the wings of these butterflies, due to the incident ion beam, is successfully neutralized, leading to images displaying a large depth-of-field and a high level of surface detail, which would normally be obscured by traditional coating methods used for scanning electron microscopy (SEM). The images are compared with those from variable pressure SEM, demonstrating the superiority of HIM at high magnifications. In addition, the large depth-of-field capabilities of HIM are exploited through the creation of stereo pairs that allows the exploration of the third dimension. Furthermore, the extraction of quantitative height information which matches well with cross-sectional transmission electron microscopy measurements from the literature is demonstrated. SCANNING 33: 107120, 2012. (c) 2011 Wiley Periodicals, Inc.