EPIDERMAL GROWTH-FACTOR RECEPTOR AND TRANSFORMING GROWTH-FACTOR ALPHA EXPRESSION IN HUMAN OVARIAN CARCINOMAS
EPIDERMAL GROWTH-FACTOR RECEPTOR AND TRANSFORMING GROWTH-FACTOR ALPHA EXPRESSION IN HUMAN OVARIAN CARCINOMAS
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DOI：
10.1016/0959-8049(92)90538-d
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发表时间：
1992-01-01
影响因子：
8.4
通讯作者：
WAGNER, E
中科院分区：
文献类型：
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作者：
KOHLER, M;BAUKNECHT, T;WAGNER, E
卵巢癌不同的肿瘤生物学行为可能影响肿瘤的可操作性和对化疗的反应，这是最相关的预后因素之一。由于认为可能与表皮生长因子/转化生长因子α (EGF/ tgf - α)信号通路的激活有关，我们采用分子化学、生化和免疫组织化学方法分析了42例卵巢癌、4例卵巢转移瘤、2例其他恶性卵巢肿瘤和25例非恶性组织（卵巢、子宫肌层）中表皮生长因子受体（EGFR）和tgf - α的表达。未发现EGFR或tfg - α基因的重大重排或扩增。在非恶性组织中未检测到强烈的EGFR或tgf - α信号。免疫组化结果显示，tgf - α主要由肿瘤细胞产生。western blot分析在恶性组织中检测到4种不同的高分子量形式（20 ~ 48 kD）。
The varying tumorbiological behaviour of ovarian carcinomas probably influences operability, response to chemotherapy, being one of the most relevant prognostic factors. Because it is believed that an activation of the epidermal growth factor/transforming growth factor alpha (EGF/TGF-alpha) signal pathway could be involved, we analysed the expression of epidermal growth factor receptor (EGFR) and TFG-alpha with molecular-chemical, biochemical and immunohistochemical methods in 42 ovarian carcinomas, 4 ovarian metastasis, 2 other malignant ovarian tumours, and in 25 nonmalignant tissues (ovary, myometrium). No major rearrangements or amplification of the EGFR or TFG-alpha genes were found. In non-malignant tissues no strong EGFR or TGF-alpha signals were detected. TGF-alpha is mainly produced by the tumour cells as shown by immunohistochemistry. Four different high molecular weight forms (20-48 kD) were detected in malignant tissues by western blot analysis.