Daun02 Inactivation of Behaviorally Activated Fos-Expressing Neuronal Ensembles.
Daun02 Inactivation of Behaviorally Activated Fos-Expressing Neuronal Ensembles.
复制标题
DOI：
10.1002/cpns.2
复制
发表时间：
2016-07-01
影响因子：
--
通讯作者：
Hope, Bruce T
中科院分区：
文献类型：
--
作者：
Koya, Eisuke;Margetts-Smith, Gabriella;Hope, Bruce T
关于显著经验（例如药物暴露，压力）及其相关环境刺激的学习关联由少数稀疏分布的行为激活神经元介导，这些神经元被称为“神经元合奏”。多年来，人们不知道这些神经元集合是否参与介导学习行为。然而，在过去的几年中，在Fos-lacZ转基因大鼠中的“Daun 02失活技术”已被证明在表达活性调节蛋白“Fos”和学习行为的神经元集合之间建立因果关系方面非常有用。这些大鼠中激活的Fos表达神经元也表达细菌蛋白β-半乳糖苷酶（β-gal）。当前药Daun 02在行为（例如药物寻求）或线索暴露后90分钟注射到这些大鼠的脑中时，Daun 02被β-gal转化为柔红霉素，β-gal使Fos和β-gal表达神经元失活。本单元介绍了饲养Fos-lacZ大鼠和进行适当Daun 02灭活实验的方案。
Learned associations about salient experiences (e.g. drug exposure, stress) and their associated environmental stimuli are mediated by a minority of sparsely distributed, behaviourally activated neurons coined ‘neuronal ensembles’. For many years, it was not known whether these neuronal ensembles were involved in mediating learned behaviours. However, in the last several years the ‘Daun02 inactivation technique’ in Fos-lacZ transgenic rats has proved very useful in establishing causal links between neuronal ensembles that express the activity-regulated protein ‘Fos’ and learned behaviours. Activated, Fos-expressing neurons in these rats also express the bacterial protein β-galactosidase (β-gal). When the prodrug Daun02 is injected into the brains of these rats 90 min after a behaviour (e.g. drug-seeking) or cue exposure, then the Daun02 is converted into daunorubicin by β-gal that inactivates the Fos and β-gal-expressing neurons. This unit presents protocols for breeding the Fos-lacZ rats and conducting appropriate Daun02 inactivation experiments.