Characterization of H5N1 influenza A viruses isolated during the 2003-2004 influenza outbreaks in Japan
Characterization of H5N1 influenza A viruses isolated during the 2003-2004 influenza outbreaks in Japan
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DOI：
10.1016/j.virol.2004.11.016
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发表时间：
2005-02-05
期刊：
影响因子：
3.7
通讯作者：
Yamaguchi, S
中科院分区：
文献类型：
--
作者：
Mase, M;Tsukamoto, K;Yamaguchi, S
在日本，2003年12月底至2004年3月期间，相距150至450公里的三个县发生了四次急性、高传染性和致命性禽类疾病暴发，涉及三个养鸡场和一群作为宠物饲养的鸡。每次疫情爆发的原因都是 H5N1 甲型流感病毒，这是自 1925 年以来在日本疫情中分离出的第一种高致病性病毒。H5N1 病毒也是从死乌鸦中分离出来的，这些乌鸦显然是通过接触受病毒污染的材料而感染的。这些 H5N1 病毒在抗原上彼此相似，但可以通过在血凝抑制测定中使用一组单克隆抗体与其他 H5 病毒（包括 1997 年和 2003 年从香港分离的病毒）区分开来。从基因上看，日本的H5N1病毒在所有基因上都密切相关，并且与2003年在中国大陆广东省分离出的V基因型单一分离株（A/chickeni/Shantou/4231/2003）有密切的遗传关系。在鸡和小鼠中研究了指示分离株（A/chicken/Yamaguchi/7/2004）的毒力。静脉内或鼻内接种该分离株的鸡分别在接种后 1 或 3 天内死亡。在小鼠中，虽然这种病毒在没有事先适应的情况下在肺部复制良好并扩散到大脑，但对50%小鼠致死的剂量为5 x 10(5) 50%鸡蛋感染剂量（EID50），其致病性低于从人类分离的香港1997年H5N1病毒。我们的研究结果表明，与日本鸡流感暴发相关的H5N1病毒在基因型上与2003年从中国鸡中分离出的H5N1病毒（基因型V）密切相关，但与2003-2004年东南亚流行的病毒（即基因型Z）不同，并且这些高致病性病毒可以传播给对这些病毒高度敏感的乌鸦。 (C) 2004 Elsevier Inc. 保留所有权利。
In Japan, between the end of December 2003 and March 2004, four outbreaks of acute, highly transmissible and lethal disease occurred in birds in three prefectures separated by 150-450 km, involving three chicken farms and a group of chickens raised as pets. The cause of each outbreak was an H5N1 influenza A virus-the first highly pathogenic virus to be isolated from the outbreaks in Japan since 1925. The H5N1 virus was also isolated from dead crows, apparently infected by contact with virus-contaminated material. These H5N1 viruses were antigenically similar to each other, but could be differentiated from other H5 viruses, including those isolated from Hong Kong in 1997 and 2003, by use of a panel of monoclonal antibodies in hemagglutination inhibition assays. Genetically, the H5N1 viruses in Japan were closely related to each other in all genes and were genetically closely related to a single isolate of genotype V that was isolated in 2003 in the Guandong Province of mainland China (A/chickeni/Shantou/4231/2003). The virulence of the index isolate (A/chicken/Yamaguchi/7/2004) was studied in chickens and mice. Chickens intravenously or intranasally inoculated with the isolate died within 1 or 3 days of inoculation, respectively. In mice, although this virus replicated well in the lung without prior adaptation and spread to the brain, the dose lethal to 50% of the mice was 5 x 10(5) 50% egg infectious doses (EID50), which is less pathogenic than the Hong Kong 1997 H5N1 viruses isolated from humans. Our findings indicate that the H5N1 viruses associated with the influenza outbreaks in chickens in Japan were genotypically closely related to an H5N1 virus isolated from chicken in China in 2003 (genotype V), but were different from those prevalent in southeastern Asia in 2003-2004 (i.e., genotype Z) and that these highly pathogenic viruses can be transmitted to crows, which are highly susceptible to these viruses. (C) 2004 Elsevier Inc. All rights reserved.