Cytolysin-mediated translocation (CMT): A functional equivalent of type III secretion in Gram-positive bacteria
Cytolysin-mediated translocation (CMT): A functional equivalent of type III secretion in Gram-positive bacteria
复制标题
DOI：
10.1016/s0092-8674(01)00198-2
复制
发表时间：
2001-01-12
期刊：
影响因子：
64.5
通讯作者：
Caparon, M
中科院分区：
文献类型：
--
作者：
Madden, JC;Ruiz, N;Caparon, M
尽管革兰氏阳性菌在疾病中很重要，但尚未描述用于将效应蛋白注射到宿主细胞中的 III 型分泌。在这里，我们描述了革兰氏阳性病原体化脓性链球菌的注射途径，该途径利用链球菌溶血素 O (SLO)，一种胆固醇依赖性溶细胞素。该数据支持一个模型，其中效应器通过极化过程通过 SLO 孔移位。效应器 SPN（化脓性链球菌 NAD-糖水解酶）能够产生有效的第二信使环状 ADP-核糖，SLO 和 SPN 协同作用以触发细胞毒性。这些数据为胆固醇依赖性溶细胞素家族的功能提供了一个新的范例，其广泛分布表明溶细胞素介导的易位（CMT）可能相当于革兰氏阳性病原体的 III 型分泌。
Type III secretion for injection of effector proteins into host cells has not been described for Gram-positive bacteria despite their importance in disease. Here, we describe an injection pathway for the Gram-positive pathogen Streptococcus pyogenes that utilizes streptolysin O (SLO), a cholesterol-dependent cytolysin. The data support a model in which an effector is translocated through the SLO pore by a polarized process. The effector, SPN (S. pyogenes NAD-glycohydrolase), is capable of producing the potent second messenger cyclic ADP-ribose, and SLO and SPN act synergistically to trigger cytotoxicity. These data provide a novel paradigm for the function of the cholesterol-dependent cytolysin family and its wide distribution suggests that cytolysin-mediated translocation (CMT) may be the equivalent of type III secretion for Gram-positive pathogens.