Vaccination against tuberculosis by DNA injection
Vaccination against tuberculosis by DNA injection
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DOI：
10.1038/nm0896-888
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发表时间：
1996-08-01
期刊：
影响因子：
82.9
通讯作者：
Lowrie, DB
中科院分区：
文献类型：
--
作者：
Tascon, RE;Colston, MJ;Lowrie, DB
全世界每年有300万人死于结核病，而艾滋病使这一问题更加严重。需要一种比目前使用的活分枝杆菌卡介苗(BCG)更好的疫苗。当小鼠被注射编码单个分枝杆菌抗原(65-kDa热休克蛋白，hsp65)的质粒DNA时，它们对该蛋白产生特异性的细胞和体液反应，并对随后的结核分枝杆菌攻击产生免疫力。免疫效果相当于接种活的卡介苗，而用该蛋白免疫则无效。编码另一种分枝杆菌抗原(36 kDa富含脯氨酸的抗原)的DNA也获得了保护。这些结果表明，DNA疫苗可能产生更好的疫苗来取代卡介苗。
There are 3 million deaths per annum worldwide due to tuberculosis, and AIDS is compounding the problem. A better vaccine than the live mycobacterium currently in use, bacillus Calmette-Guerin (BCG), is needed. When mice were injected with plasmid DNA encoding a single mycobacterial antigen (65-kDa heat shock protein, hsp65) they made specific cellular and humoral responses to the protein and became immune to subsequent challenge with Mycobacterium tuberculosis. Protection was equivalent to that obtained by vaccinating with live BCG, whereas immunizing with the protein was ineffective. Protection was also obtained with DNA encoding another mycobacterial antigen (36-kDa proline-rich antigen). These results suggest that DNA vaccination might yield improved vaccines to replace BCG.