Safety assessment and probiotic evaluation of Enterococcus faecium YF5 isolated from sourdough.
Safety assessment and probiotic evaluation of Enterococcus faecium YF5 isolated from sourdough.
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DOI：
10.1111/1750-3841.12079
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发表时间：
2013-04
影响因子：
3.9
通讯作者：
Q. Tan;Hengyi Xu;Zoraida P. Aguilar;Shanshan Peng;Suqin Dong;Baogui Wang;Ping-Chen Li;Tingtao Chen-Tingtao-Ch
中科院分区：
文献类型：
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作者：
Q. Tan;Hengyi Xu;Zoraida P. Aguilar;Shanshan Peng;Suqin Dong;Baogui Wang;Ping-Chen Li;Tingtao Chen-Tingtao-Ch
屎肠球菌YF 5，以前从酸面团中分离的菌株，进行了安全性和益生菌潜力评估。对其毒力和耐药表型（溶细胞素和明胶酶的产生、抗生素敏感性）和基因（cylA、gelE、ace、agg、esp和vanA）进行了调查。结果表明，供试毒力因子无毒。此外，E. YF 5对阿莫西林、万古霉素、氯霉素3种抗生素敏感。此外，还进行了E. YF 5研究与对照组相似，表明无异常。此外，E.粪菌YF 5在体外低pH、胆盐、胃液和肠液中能稳定存活。此外，E.粪菌YF 5对人结肠癌细胞HT-29的粘附率为3.39（±0.67）× 10（5）CFU/mL。当与病原微生物共培养时（坂崎肠杆菌CMCC 45402、大肠杆菌CMCC 44102、肠出血性大肠杆菌O 157：H7 CMCC 44828、鼠伤寒沙门氏菌CMCC 50071、福氏志贺菌301和宋内志贺菌ATCC 29930）和2株革兰氏阳性菌株（单核细胞增生李斯特菌CMCC 54001和金黄色葡萄球菌CMCC 26003），除S.金黄色。因此，E.屎肠菌YF 5可以被认为是一种安全的菌株，并且它可以用作益生菌制剂或用于微生态制剂。
Enterococcus faecium YF5, a strain previously isolated from sourdough, was assessed for safety and probiotic potential. Its virulence and antibiotic resistant phenotypes (cytolysin and gelatinase production, antibiotic susceptibility) and genes (cylA, gelE, ace, agg, esp, and vanA) were surveyed. Results indicated that the tested virulence determinants were nontoxic. In addition, E. faecium YF5 was sensitive to 3 antibiotics such as amoxicillin, vancomycin, and chloramphenicol. Furthermore, results of in vivo animal acute oral toxicity of E. faecium YF5 studies were similar to the control group that indicated no abnormalities. In addition, E. faecium YF5 stably survived in low pH, bile salts, gastric, and intestinal fluids in vitro. Moreover, E. faecium YF5 was found to adhere to human colon cancer cell line HT-29 at 3.39 (±0.67) × 10(5) CFU/mL. When cocultured with pathogenic organisms (Enterobacter sakazakii CMCC45402, Escherichia coli CMCC44102, enterohemorrhage Escherichia coli O157: H7 CMCC44828, Salmonella Typhimurium CMCC50071, Shigella flexneri 301, and Shigella sonnei ATCC 29930) and 2 gram-positive strains (Listeria monocytogenes CMCC54001 and Staphylococcus aureus CMCC 26003), it inhibited these foodborne pathogens with exception of S. aureus. Therefore, E. faecium YF5 can be regarded as a safe strain and it may be used as a probiotic preparation or for microecologics.