The occurrence of antibodies in rabbit anti-pneumococcus antisera directed against calcium dependent and independent determinants.
The occurrence of antibodies in rabbit anti-pneumococcus antisera directed against calcium dependent and independent determinants.
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兔抗肺炎球菌抗血清中出现针对钙依赖性和独立决定簇的抗体。
DOI：
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发表时间：
1975
期刊：
影响因子：
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通讯作者：
P. Maurer
中科院分区：
文献类型：
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作者：
H. Callahan;P. Maurer
来自肺炎球菌的两种类型的特异性酸性多糖（III型和VIII型）已被用于制备免疫吸附剂。多糖通过 1,6 己二胺间隔基与 Sepharose 4b 偶联，用于从兔抗肺炎球菌抗血清中分离出两组类型特异性抗体。一个群体需要钙来影响与同源多糖的相互作用，而另一群体则没有这样的要求。分离的抗体属于 IgG 类，在沉淀素测定中具有完全活性。
Two type specific acidic pol-saccharides from Pneumococci (type III and type VIII) have been used to prepare immuno-adsorbents. The polysaccharides were coupled to Sepharose 4b via a 1,6 hexanediamine spacer and used to isolate two populations of type specific antibodies from rabbit anti-pneumococcus antisera. One population requires calcium to affect interaction with the homologous polysaccharide, while the other population has no such requirement. The isolated antibodies are of the lgG class and are fully active in precipitin assays.