Multiple Amino Acid-Excluded Genetic Codes for Protein Engineering Using Multiple Sets of tRNA Variants
Multiple Amino Acid-Excluded Genetic Codes for Protein Engineering Using Multiple Sets of tRNA Variants
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DOI：
10.1021/sb400144h
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发表时间：
2014-03-01
影响因子：
4.7
通讯作者：
Kiga, Daisuke
中科院分区：
文献类型：
--
作者：
Amikura, Kazuaki;Sakai, Yoko;Kiga, Daisuke
最近开发了一种“简化遗传密码”，只有19个氨基酸分配给正义密码子。在这项研究中，我们描述了新的简化代码，其中多个氨基酸同时排除通用代码。在最简单的密码中，色氨酸、半胱氨酸、酪氨酸和天冬酰胺密码子通过四种tRNA变体被分配给丝氨酸。结果表明，不同的氨基酸组可以很容易地从通用密码中排除，使用我们的遗传密码简化策略。一个简化的遗传密码是有用的工程工具，用于改善工业酶和药物，也提供了新的见解评估蛋白质进化。其中多个氨基酸同时从代码中排除的简化代码可以是比仅排除一个氨基酸的代码更有效的工具。
A "simplified genetic code", with only 19 amino acids assigned to the sense codons, was recently developed. In this study, we describe novel simplified codes in which multiple amino acids are simultaneously excluded from the universal code. In the simplest code, tryptophan, cysteine, tyrosine, and asparagine codons are assigned to serine by using four kinds of tRNAs" variants. The results revealed that various sets of amino acids can easily be excluded from the universal code, using our strategy for genetic code simplification. A simplified genetic code is useful as an engineering tool for the improvement of industrial enzymes and pharmaceuticals, and also provides new insights into the assessment of protein evolution. Simplified codes in which multiple amino acids are simultaneously excluded from the code can be more effective tools than codes excluding only one amino acid.