Helicobacter pylori eradication induces marked increase in H+/K+- adenosine triphosphatase expression without altering parietal cell number in human gastric mucosa
Helicobacter pylori eradication induces marked increase in H+/K+- adenosine triphosphatase expression without altering parietal cell number in human gastric mucosa
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DOI：
10.1136/gut.2005.066464
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发表时间：
2006-02-01
期刊：
影响因子：
24.5
通讯作者：
Sugano, K
中科院分区：
文献类型：
--
作者：
Osawa, H;Kita, H;Sugano, K
背景和目标：幽门螺杆菌感染后胃酸分泌下调，根除后胃酸分泌上调，但其机制尚不清楚。我们研究了幽门螺杆菌根除对壁细胞的数量和在人胃mucosa.Methods酸分泌功能的分子的表达的影响：我们连续招募了111名男性慢性胃炎诱导幽门螺杆菌。在成功根除幽门螺杆菌之前和之后12周通过内窥镜获得活检标本，并计数壁细胞数量。采用真实的时间逆转录聚合酶链反应方法测定H +/K +-ATP酶、阴离子交换体2、M3毒蕈碱受体、内因子和白细胞介素1 β的mRNA表达水平。胃萎缩的严重程度进行了评估，使用血清胃蛋白酶原I/II ratio. Results：没有显着差异，观察壁细胞数量在幽门螺杆菌根除前后。幽门螺杆菌根除后，胃粘膜H +/K +-ATP酶的中位mRNA表达水平增加了250倍，同时伴有白细胞介素1 β的减弱。H~+/K~+-ATP酶的表达在重度萎缩性胃炎患者中也有明显增加。相比之下，根除治疗后，包括内因子、阴离子交换剂2和M3毒蕈碱受体在内的mRNA表达水平的倍数增加分别限制在1.4、2.3和2.5倍。在壁细胞数量没有改变的情况下，成功根除幽门螺杆菌后，胃H +/K +-ATP酶mRNA表达显著恢复，提示H +/K +-ATP酶表达的恢复在幽门螺杆菌根除后胃酸分泌恢复中的中心作用。
Background and aims: Gastric acid secretion is downregulated by Helicobacter pylori infection and upregulated after its eradication, but the mechanisms are still unclear. We examined the effects of H pylori eradication on the number of parietal cells and on expression of molecules functioning in acid secretion in the human gastric mucosa.Methods: We enrolled 111 consecutive men with chronic gastritis induced by H pylori. Biopsy specimens were endoscopically obtained before and 12 weeks after successful eradication of H pylori and parietal cell numbers were counted. mRNA expression levels of H+/K+-adenosine triphosphatase ( H+/K+-ATPase), anion exchanger 2, M3 muscarinic receptor, intrinsic factor, and interleukin 1 beta were determined with a real time reverse transcriptase-polymerase chain reaction method. The severity of gastric atrophy was evaluated using the serum pepsinogen I/II ratio.Results: No significant difference was observed in parietal cell numbers before and after H pylori eradication. Median mRNA expression levels of H+/K+-ATPase in the gastric mucosa increased 250-fold after H pylori eradication accompanied by attenuation of interleukin 1 beta. A large increase in H+/K+-ATPase expression was observed even in patients with severe atrophic gastritis. In contrast, fold increases in mRNA expression levels, including intrinsic factor, anion exchanger 2, and M3 muscarinic receptor, after eradication therapy, were limited to 1.4, 2.3, and 2.5 times, respectively.Conclusions: In the absence of alteration of parietal cell number, gastric H+/K+-ATPase mRNA expression was markedly restored after successful H pylori eradication, suggesting a central role for the restoration of H+/K+-ATPase expression in gastric acid secretion recovery after H pylori eradication.