A new type of papillomavirus associated with cancer of the uterine cervix.
A new type of papillomavirus associated with cancer of the uterine cervix.
复制标题
一种与宫颈癌相关的新型乳头瘤病毒。
DOI：
10.1016/0042-6822(87)90366-7
复制
发表时间：
1987
期刊：
影响因子：
3.7
通讯作者：
Temple,GF
中科院分区：
文献类型：
--
作者：
Lorincz,AT;Quinn,AP;Lancaster,WD;Temple,GF
对一例宫颈内膜腺癌组织DNA进行低严密Southern杂交分析，发现一种新的人乳头状瘤病毒(HPV)。将病毒基因组片段克隆到λL47中，并将其亚克隆到pBR322中进行进一步鉴定。杂交研究表明，这些病毒DNA分离株与其他HPV只有较远的亲缘关系，因此代表了一种新的HPV类型，称为HPV35。构建了HPV的限制性内切酶图谱，比较了HPV和HPV6b的遗传结构，结果表明HPV35和HPV6b基因组具有共线性。患病率研究表明，在158例肛门生殖器上皮内瘤变中有2例(1%)存在HPV35，在69例肛门癌中有3例(4%)存在HPV35。因此，HPV35是一种与肛门上皮内瘤变和癌症相关的低流行率的人乳头瘤病毒。
A new human papillomavirus (HPV) type was detected by low-stringency Southern blot hybridization analysis of DNA from an endocervical adenocarcinoma. The genomic DNA of the virus, which was obtained as twoBamHI fragments of 3.75 and 4.1 kb, was molecularly cloned into λL47 and subsequently subcloned into pBR322 for further characterization. Hybridization studies demonstrated that these viral DNA isolates were only distantly related to other HPV and thus represented a new type of HPV, called HPV 35. A restriction enzyme map was prepared which allowed a comparison of the genetic organization of this HPV with that of HPV 6b; the results demonstrated collinearity of the HPV 35 genome with that of HPV 6b. Prevalence studies revealed that HPV 35 was present in 2 of 158 (1%) anogenital intraepithelial neoplasia and in 3 of 69 (4%) anogenital cancers. Thus HPV 35 is a low-prevalence human papillomavirus associated with anogenital intraepithelial neoplasia and cancer.