The Recovery of Mice from Influenza A Virus Infection: Adoptive Transfer of Immunity with Influenza Virus‐specific Cytotoxic T Lymphocytes Recognizing a Common Virion Antigen
The Recovery of Mice from Influenza A Virus Infection: Adoptive Transfer of Immunity with Influenza Virus‐specific Cytotoxic T Lymphocytes Recognizing a Common Virion Antigen
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小鼠从甲型流感病毒感染中恢复：识别常见病毒颗粒抗原的流感病毒特异性细胞毒性 T 淋巴细胞的过继免疫转移
DOI：
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发表时间：
1978
影响因子：
3.7
通讯作者：
G. Ada
中科院分区：
文献类型：
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作者：
K. L. Yap;G. Ada
鼻内接种给定 A 株传染性流感病毒的小鼠在 24 小时后被过继转移，并使用体外或完全体内产生的二次流感病毒免疫 T 细胞制剂。免疫细胞是在同源或异源A株流感病毒或B型病毒感染期间产生的。如果使用同源病毒，则会产生最大的抗病毒效果（通过受体小鼠肺部病毒水平的降低来衡量）。血凝素特异性的共享被证明是重要的，但如果血凝素和神经氨酸酶抗原特异性都不共享，则仍然表达显着的抗病毒活性。抗病毒作用具有类型特异性。 A型流感免疫T细胞的过继转移不表达针对B型病毒的抗病毒活性，反之亦然。在早期工作的基础上，转移细胞中的效应细胞群是细胞毒性T细胞（Tc）。用与第一个感染病毒既不具有血凝素也不具有神经氨酸酶抗原特异性的异源 A 型病毒进行鼻内再感染，会诱导针对 A 型流感病毒的交叉反应性 Tc 的增强和更早产生。与此同时，肺部病毒水平也显着降低。这项工作的结果证明了小鼠流感病毒感染中的异型细胞介导的免疫。
Mice inoculated intranasally with infectious influenza virus of a given A strain were adoptively transferred 24 h later with preparations of secondary influenza virus‐immune T cells generated either in vitro or entirely in vivo. The immune cells were raised during infection with homologous or heterologous A strain influenza viruses or with a type B virus. The greatest antiviral effect, measured by reduction in lung virus level of recipient mice, occurred if homologous viruses were used. Sharing of haemagglutinin specificity was shown to be important, but significant antiviral activity was still expressed if neither haemagglutinin nor neuraminidase antigenic specificities were shared. The antiviral effect was type‐specific. Adoptive transfer of type A influenza immune T cells did not express antiviral activity against type B virus, and vice versa. On the basis of earlier work, the effector population in the transferred cells was cytotoxic T cells (Tc). Intranasal reinfection of mice with a heterologous type A virus sharing neither haemagglutinin nor neuraminidase antigenic specificity with the first infecting virus induced enhanced and earlier production of cross‐reactive Tc against type A influenza viruses. This was paralleled by significantly lower virus levels in the lungs. The results of this work demonstrate heterotypic cell‐mediated immunity in influenza virus infection in mice.