NEURAL FOLD AND NEURAL CREST MOVEMENT IN THE MEXICAN SALAMANDER AMBYSTOMA-MEXICANUM
NEURAL FOLD AND NEURAL CREST MOVEMENT IN THE MEXICAN SALAMANDER AMBYSTOMA-MEXICANUM
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DOI：
10.1002/jez.1402340108
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发表时间：
1985-01-01
影响因子：
--
通讯作者：
BRUN, RB
中科院分区：
文献类型：
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作者：
BRUN, RB
在两栖动物神经发育的研究中，术语神经脊、神经褶和神经嵴有时用作同义词。这有时会导致误解，认为神经嵴移植等同于神经皱襞移植。神经襞由三部分组成：神经嵴、未来神经管组织和表皮。为了研究这些神经褶皱组件在神经形成过程中如何移动，进行了延时摄影、电镜和移植。在神经形成期间定期对墨西哥钝口螈胚胎进行拍照。对照片进行分析，找出神经形成开始时这些细胞的位置，当神经折叠关闭时，这些细胞最终位于融合线上。之后，这些细胞已经位于新兴的神经褶上。在前神经皱襞中，这些细胞位于外侧表皮。神经皱襞的电镜证实了表皮的存在。为了跟踪分化为黑素细胞（神经嵴）的细胞的运动，神经褶部分被移植到白化宿主中。异位移植后分化为黑素细胞的嵴细胞位于与神经板接触的神经皱襞的侧翼。在来自外侧（远端）侧翼的移植物中，没有形成黑素细胞。半薄切片显示神经皱襞的第三部分由已知可分化为神经组织的顶端收缩细胞组成。由于神经襞由表皮、神经组织和神经嵴组成，因此神经襞和神经嵴不能作为同义词使用。
In studies of amphibian neurulation, the terms neural ridge, neural fold and neural crest are sometimes used as synonyms. This has occasionally led to the misconception that grafting of the neural crest is equivalent to grafting of the neural fold. The neural fold is composed of 3 parts: the neural crest, prospective neural tube tissue and epidermis. To investigate how these neural fold components move during neurulation, time-lapse photpgraphy, EM and grafting were performed. Ambystoma mexicanum embryos were photographed during neurulation at regular intervals. The photographs were analyzed to find the position of those cells at beginning of neurulation that end up on the line of fusion as the neural folds close. Posteriorly, these cells are already on the emerging neural fold. In the anterior neural folds, these cells are located in the lateral epidermis. EM of the neural folds confirms the presence of epidermis. To follow the movement of the cells differentiating into melanophores (neural crest), neural fold parts were grafted into albino hosts. The crest cells differentiating into melanophores following ectopic grafting are located in the flank of the neural fold that is in contact with the neural plate. In grafts from the outside (distal) flank, no melanophores developed. Semithin sections show that the 3rd part of the neural fold consists of apically constricted cells known to differentiate into neural tissue. Because the neural folds consist of epidermis, neural tissue and neural crest, neural fold and neural crest cannot be used as synonyms.