SOME MINOR COMPONENTS OF BACTERIOPHAGE-T2 PARTICLES
SOME MINOR COMPONENTS OF BACTERIOPHAGE-T2 PARTICLES
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DOI：
10.1016/0042-6822(57)90061-2
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发表时间：
1957-01-01
期刊：
影响因子：
3.7
通讯作者：
HERSHEY, AD
中科院分区：
文献类型：
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作者：
HERSHEY, AD
纯化的噬菌体标记的葡萄糖，赖氨酸，或精氨酸放射性碳与三氯乙酸和水解产物的馏分进行了纸层析和放射性分析。在酸溶性组分中发现了两种以前未检出的组分：含有赖氨酸、天冬氨酸和谷氨酸的“酸溶性肽”，以及标记为精氨酸但不含精氨酸的未鉴别的可透析“物质A”。这两种物质各自含有噬菌体颗粒总碳的大约1%。当标记的底物被喂给受感染的细菌，在氯霉素的存在下合成脱氧核糖核酸，并允许噬菌体颗粒形成后，随后去除氯霉素和放射性底物，颗粒含有标记的核酸和物质A，但没有标记的多肽。因此，氯霉素抑制肽的形成，但不抑制核酸合成或精氨酸转化为物质A。以上述方式标记的噬菌体颗粒也用于研究从亲本噬菌体到后代噬菌体的同位素转移。核酸和物质A，而不是多肽，被转移。物质A的转移似乎与核酸的转移无关，如放射性物质的喂养实验和非放射性物质的同位素竞争试验所示。因此，没有证据表明在生长过程中核酸和其他噬菌体前体之间存在关联。
Purified phage labeled with glucose, lysine, or arginine radiocarbon was fractionated with trichloroacetic acid and hydrolysates of the fractions were analyzed by paper chromatography and radioassay. Two previously undetected components were found in the acid soluble fraction: an "acid-soluble peptide" containing lysine, aspartic acid, and glutamic acid, and an unidentified dialysable "substance A" that is labeled from arginine but does not contain arginine. The two substances each contain roughly 1% of the total carbon of the phage particles. When labeled substrates were fed to infected bacteria synthesizing deoxyribo-nucleic acid in the presence of chloramphenicol, and phage particles were allowed to form after subsequent removal of both chloramphenicol and radioactive substrate, the particles contained labeled nucleic acid and substance A but not labeled polypeptides. Thus chloramphenicol inhibits peptide formation, but not nucleic acid synthesis or conversion of arginine to substance A. Phage particles labeled in the ways mentioned were also used to study isotope transfer from parental to offspring phage. Nucleic acid and substance A, but not polypeptides, are transferred. The transfer of substance A appears to be independent of that of nucleic acid, as shown by feeding experiments with the radioactive substance, and isotope competition tests with the nonradioactive substance. Thus no evidence could be found for association between nucleic acid and other phage precursors during growth.