Production of Biosurfactant Lipopeptides Iturin A, Fengycin, and Surfactin A from Bacillus subtilis CMB32 for Control of Colletotrichum gloeosporioides
Production of Biosurfactant Lipopeptides Iturin A, Fengycin, and Surfactin A from Bacillus subtilis CMB32 for Control of Colletotrichum gloeosporioides
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DOI：
10.4014/jmb.0905.05007
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发表时间：
2010-01-01
影响因子：
2.8
通讯作者：
Chl, Youn-Tae
中科院分区：
文献类型：
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作者：
Kim, Pyoung Il;Ryu, Jaewon;Chl, Youn-Tae
从土壤中分离出的一种菌株，因其具有控制胶孢炭疽病引起的炭疽病的潜力，被鉴定为枯草芽孢杆菌。枯草芽孢杆菌 CMB32 在 30 ℃ 的 M9 肉汤中产生抗真菌剂。将枯草芽孢杆菌 CMB32 产生的生物表面活性剂脂肽通过调节 pH 至 2 并使用氯仿/甲醇萃取进行沉淀，然后使用柱色谱和反相 HPLC 进行纯化。通过 MALDI-TOF 质谱法估计脂肽的分子量分别为 (a) 1,080、(b) 1,486 和 (c) 1,044 Da。它们分别具有(a) Pro、Asx、Ser、Tyr、Glx，(b) Glx、Tyr、Ala、Pro、Ile 和(c) Glx、Leu、Val、Asx 的环状结构和氨基酸组成。进一步分析表明，枯草芽孢杆菌 CMB32 产生三种抗真菌脂肽：(a) iturin A、(b) fengycin 和 (c) surfactin A。
A bacterial strain isolated from soil for its potential to control the anthracnose disease caused by Colletotrichum gloeosporioides was identified as a Bacillus subtilis. Bacillus subtilis CMB32 produced antifungal agents on M9 broth at 30 degrees C. Biosurfactant lipopeptides produced by Bacillus subtilis CMB32 were precipitated by adjusting to pH 2 and extracting using chloroform/methanol, and then were purified using column chromatography and reverse-phase HPLC. The molecular masses of the lipopeptides were estimated by MALDI-TOF mass spectrometry as (a) 1,080, (b) 1,486, and (c) 1,044 Da, respectively. They had cyclic structures and amino acid compositions of (a) Pro, Asx, Ser, Tyr, Glx, (b) Glx, Tyr, , Ala, Pro, Ile, and (c) Glx, Leu, Val, Asx, respectively. Further analysis revealed that Bacillus subtilis CMB32 produced three antifungal lipopeptides: (a) iturin A, (b) fengycin, and (c) surfactin A.