Fibroblast growth factor 21 as a possible endogenous factor inhibits apoptosis in cardiac endothelial cells
Fibroblast growth factor 21 as a possible endogenous factor inhibits apoptosis in cardiac endothelial cells
复制标题
成纤维细胞生长因子 21 作为一种可能的内源性因子抑制心脏内皮细胞凋亡
DOI：
10.3760/cma.j.issn.0366-6999.2010.23.008
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发表时间：
2010-12-05
影响因子：
6.1
通讯作者：
Zhang Ying-chuan
中科院分区：
文献类型：
--
作者：
Lu Yun;Liu Jing-hua;Zhang Ying-chuan
成纤维细胞生长因子21 （Fibroblast growth factor 21, FGF21）是FGF超家族的新成员，是一种重要的内源性全身糖脂代谢调节因子。本研究旨在探讨FGF21是否能作为一种独立的保护因子，减轻动脉粥样硬化损伤，预防内皮功能障碍。采用real - time-PCR法检测体外培养的心肌微血管内皮细胞（CMECs）中FGF21 mRNA的表达水平，酶联免疫吸附法检测培养液中FGF21 mRNA的表达水平，分析未处理对照组和ox LDL培养的CMECs中FGF21 mRNA的表达水平。结果与ox低密度脂蛋白孵育cmec后，FGF21 mRNA和蛋白的分泌水平均显著上调，且增加200 μ mol/L贝唑菲特可减少cmec凋亡，并通过shRNA诱导凋亡抑制FGF21的表达(P
Background Fibroblast growth factor 21 (FGF21) is a new member of FGF super family that is an important endogenous regulator for systemic glucose and lipid metabolism This study aimed to explore whether FGF21 reduces atherosclerotic injury and prevents endothelial dysfunction as an independent protection factorMethods The present study was designed to investigate the changes of FGF21 levels induced by oxidized low density lipoprotein (ox LDL), and the changes of apoptosis affected by regulating FGF21 expression The FGF21 mRNA levels of cultured cardiac microvascular endothelial cells (CMECs) were determined by real time-PCR and the protein concentration in culture media was detected by enzyme linked immunosorbent assay We analyzed the different expression levels of untreated controls and CMECs incubated with ox LDL, and the changes of CMECs apoptosis initiated by the enhancement or suppression of FGF21 levelsResults The secretion levels of FGF21 mRNA and protein were significantly upregulated in CMECs incubated with ox LDL Furthermore, FGF21 levels increased by 200 mu mol/L bezafibrate could reduce CMECs apoptosis, and inhibit FGF21 expression by shRNA induced apoptosis (P