Diethyl phthalate enhances apoptosis induced by serum deprivation inPC12 cells
Diethyl phthalate enhances apoptosis induced by serum deprivation inPC12 cells
复制标题
邻苯二甲酸二乙酯增强 PC12 细胞血清剥夺诱导的细胞凋亡
DOI：
10.1111/j.1742-7843.2012.00869.x
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Kurasaki M
中科院分区：
文献类型：
--
作者：
Sun Y;Takahashi K;Saito T;Hosokawa T;Kurasaki M
In this study, to examine the mechanism of diethyl phthalate toxicity to cells, the effects of diethyl phthalate on apoptosis in a PC12 cell system were investigated by assaying apoptotic factors such as caspase‐3, Bax, cytochrome c and DNA damage. Diethyl phthalate was shown to enhance the apoptosis induced by serum deprivation according to the results of DNA electrophoresis and TUNEL signal assays, although it could not induce apoptosis itself in the cells. This enhancement was thought to be because of an increase in caspase‐3‐like activity. In addition, the expression of bax and contents of cytochrome c in the cytosol showed a tendency to increase the cells exposed to diethyl phthalate. These results indicated that diethyl phthalate, a potential endocrine disrupter, affects the apoptotic system in PC12 cells. Diethyl phthalate may enhance oxidative stress such as that induced by reactive oxygen species in PC12 cells.
