GNOM-LIKE 2, encoding an adenosine diphosphate-ribosylation factor-guanine nucleotide exchange factor protein homologous to GNOM and GNL1, is essential for pollen germination in Arabidopsis.
GNOM-LIKE 2, encoding an adenosine diphosphate-ribosylation factor-guanine nucleotide exchange factor protein homologous to GNOM and GNL1, is essential for pollen germination in Arabidopsis.
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DOI：
10.1111/j.1744-7909.2009.00858.x
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发表时间：
2009-08
影响因子：
11.4
通讯作者：
Dong-Jie Jia;Xingqin Cao;Wei Wang;Xiao-Yun Tan;Xueqin Zhang;Li-Qun Chen;D. Ye
中科院分区：
文献类型：
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作者：
Dong-Jie Jia;Xingqin Cao;Wei Wang;Xiao-Yun Tan;Xueqin Zhang;Li-Qun Chen;D. Ye
在开花植物中，雄配子通过花粉管传递给雌配子体。虽然它对植物有性生殖很重要，但对控制花粉萌发和花粉管生长的遗传机制知之甚少。在这里，我们报告了两个新的突变体，gnom-like 2-1（gnl 2 -1）和gnl 2 -2的鉴定和表征，其中花粉粒在体外和体内不能萌发。GNL 2编码与参与内体再循环和内质网-高尔基体囊泡运输的腺苷二磷酸-核糖基化因子-鸟嘌呤核苷酸交换因子、GNOME和GNL 1同源的蛋白质。它在花粉粒和花粉管中大量表达。本研究结果表明GNL 2在花粉萌发中起着重要作用。
In flowering plants, male gametes are delivered to female gametophytes by pollen tubes. Although it is important for sexual plant reproduction, little is known about the genetic mechanism that controls pollen germination and pollen tube growth. Here we report the identification and characterization of two novel mutants, gnom-like 2-1 (gnl2-1) and gnl2-2 in Arabidopsis thaliana, in which the pollen grains failed to germinate in vitro and in vivo. GNL2 encodes a protein homologous to the adenosine diphosphate-ribosylation factor-guanine nucleotide exchange factors, GNOM and GNL1 that are involved in endosomal recycling and endoplasmic reticulum-Golgi vesicular trafficking. It was prolifically expressed in pollen grains and pollen tubes. The results of the present study suggest that GNL2 plays an important role in pollen germination.