Protection from Streptococcus pneumoniae keratitis by passive immunization with pneumolysin antiserum
Protection from Streptococcus pneumoniae keratitis by passive immunization with pneumolysin antiserum
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DOI：
10.1167/iovs.07-0492
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发表时间：
2008-01-01
影响因子：
4.4
通讯作者：
Marquart, Mary E.
中科院分区：
文献类型：
--
作者：
Green, Sherrina N.;Sanders, Melissa;Marquart, Mary E.
PURPOSE. To determine whether passive immunization with pneumolysin antiserum can reduce corneal damage associated with pneumococcal keratitis.METHODS. New Zealand White rabbits were intrastromally injected with Streptococcus pneumoniae and then passively immunized with control serum, antiserum against heat-inactivated pneumolysin (HI-PLY), or antiserum against cytotoxin-negative pneumolysin (psi PLY). Slit lamp examinations (SLEs) were performed at 24, 36, and 48 hours after infection. An additional four corneas from rabbits passively immunized with antiserum against psi PLY were examined up to 14 days after infection. Colony forming units (CFUs) were quantitated from corneas extracted at 20 and 48 hours after infection. Histopathology of rabbit eyes was performed at 48 hours after infection.RESULTS. SLE scores at 36 and 48 hours after infection were significantly lower in rabbits passively immunized with HI-PLY antiserum than in control rabbits (P
