IDENTIFICATION OF 2 PROMISCUOUS T-CELL EPITOPES FROM TETANUS TOXIN
IDENTIFICATION OF 2 PROMISCUOUS T-CELL EPITOPES FROM TETANUS TOXIN
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DOI：
10.1002/eji.1830200304
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发表时间：
1990-03-01
影响因子：
5.4
通讯作者：
RZEPCZYK, CM
中科院分区：
文献类型：
--
作者：
HO, PC;MUTCH, DA;RZEPCZYK, CM
从人供体分离破伤风类毒素特异性T细胞克隆。为了确定克隆识别的T细胞表位，筛选了代表破伤风毒素的两亲性α螺旋区的30种肽诱导克隆增殖的能力。鉴定了两个T表位。这些发生在肽12和21内，并且分别具有氨基酸序列NSVDDALKIYSYFPSV和PGINGKAIHLVNNESSE。一个不寻常的特征是，两种肽都可以通过许多HLA特异性的抗原呈递细胞呈递给它们各自的T细胞克隆。对肽12的进一步研究表明，该表位长度仅为7个氨基酸，并且具有非常疏水的序列，即YSYFPSV。含有T细胞表位的肽12和21与许多不同的HLA等位基因相互作用的能力意味着它们可能在合成疫苗中作为“通用载体分子”非常有用。
Tetanus toxoid‐specific T cell clones were isolated from a human donor. To determine the T cell epitopes recognized by the clones, 30 peptides representing amphipathic alpha helical regions of the tetanus toxin were screened for ability to induce proliferation of the clones. Two T epitopes were identified. These occurred within peptides 12 and 21, and had the amino acid sequences NSVDDALINSTKIYSYFPSV and PGINGKAIHLVNNESSE, respectively. An unusual feature was that both peptides could be presented to their respective T cell clones by antigen‐presenting cells of many HLA specificities. Further investigation of peptide 12 showed that the epitope was only seven amino acids in length and had a very hydrophobic sequence, namely YSYFPSV. The ability of the T cell epitope‐containing peptides 12 and 21 to interact with many different HLA alleles means they may potentially be very useful as “universal carrier molecules” in synthetic vaccines.