Extraction, characterization and biological activity of sulfated polysaccharides from seaweed Dictyopteris divaricata
Extraction, characterization and biological activity of sulfated polysaccharides from seaweed Dictyopteris divaricata
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DOI：
10.1016/j.ijbiomac.2018.05.134
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发表时间：
2018-10-01
影响因子：
8.2
通讯作者：
Lu, Jike
中科院分区：
文献类型：
--
作者：
Cui, Yinxin;Liu, Xin;Lu, Jike
叉开网翅藻是一种重要的褐藻，具有多种生物活性。近年来越来越受到人们的重视，但对其多糖的研究却很少。研究了叉叶网蕨海藻多糖的提取工艺及其生物活性。响应面法（RSM），基于三水平，三变量Box-Behnken设计（BBD），以获得最大多糖产量的最佳组合。最佳提取工艺条件为：液料比110 mL/g，提取时间6 h，提取温度100 ℃。在此条件下，实验收率为3.05%，与预测值3.15%基本一致。DDSP的平均分子量为58.05 kDa。气相色谱分析表明，DDSP由岩藻糖、木糖、甘露糖、葡萄糖和半乳糖组成，摩尔比为4.45：2.74：1.00：2.94：1.35。生物学活性表明，DDSP在体外具有较强的抗氧化活性，并具有刺激RAW264.7细胞免疫反应的潜力。因此，DDSP可以作为功能性食品中的天然成分。(C)2018由Elsevier B. V.出版
Dictyopteris divaricata is a kind of important brown algae with many biological activities. It has been receiving more and more attention, yet there are rarely studies done on its polysaccharides. In this study, the optimum extraction and biological activity of seaweed polysaccharides from Dictyopteris divaricata (DDSP) were investigated. Response surface methodology (RSM), based on a three-level, three-variable Box-Behnken design (BBD), was employed to obtain the best possible combinations for maximum polysaccharides yield. The optimum extraction conditions were as follows: liquid-solid ratio of 110 mL/g, extraction time of 6 h and extraction temperature of 100 degrees C. Under these conditions, the experimental yield was 3.05%, which was in close agreement with the predicted value of 3.15%. The average molecular weight of DDSP was 58.05 kDa. Gas chromatograph (GC) results showed that DDSP was composed of fucose, xylose, mannose, glucose and galactose with the corresponding molar ratio of 4.45:2.74:1.00:2.94:1.35. Biological activity showed that DDSP exhibited strong antioxidant activity in vitro and possessed the potential on stimulating immune response of RAW264.7 cells. So DDSP can be used as a natural ingredient in functional foods. (C) 2018 Published by Elsevier B.V.