Conserved pattern of OTP-positive cells in the paraventricular nucleus and other hypothalamic sites of tetrapods
Conserved pattern of OTP-positive cells in the paraventricular nucleus and other hypothalamic sites of tetrapods
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DOI：
10.1016/j.brainresbull.2007.10.037
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发表时间：
2008-03-18
影响因子：
3.8
通讯作者：
Puelles, Luis
中科院分区：
文献类型：
--
作者：
Bardet, Sylvia M.;Martinez-de-la-Torre, Margaret;Puelles, Luis
室旁核复合体（Paraventricular nucleus complex，Pa）是中枢神经回路的一个组成部分，它调节着几个稳态变量。室旁核由投射到垂体后叶的大细胞神经元和投射到中枢神经系统许多部位的小细胞神经元组成。根据修正的前体模型，室旁核位于眼柄的尾侧，沿着下丘脑背侧背板的吻尾向。尾侧，室旁核邻接前丘脑（原体3），整个复合体的腹侧和背侧由Dlx 5表达结构域的背板。同源结构域转录因子Orthopedia（Otp）在几个单独的下丘脑部位表达：室旁核、乳头周围区和弓状核。在这项研究中，我们比较了OTP在小鼠（小家鼠），鸡（原鸡），青蛙（蛙perezi）和axolotol（Ambystoma mexicanum）下丘脑的表达，采用免疫组织化学和原位杂交技术。在所有情况下，室旁核中的Otp阳性细胞被排除在Dlx5表达的相邻区域之外。在弓状核和斜乳头周围带中观察到其他阳性神经元群体。在内侧杏仁核的表达似乎是连续的OTP表达室旁核复合体。这个区域在两栖动物的大脑中相对不外翻，在小鸡中几乎没有外翻，在老鼠中完全外翻。这些数据使我们得出结论，OTP的表达模式是拓扑高度保守的四足动物和近晶脊索动物之间。(c)2007爱思唯尔公司All rights reserved.
The paraventricular nucleus complex (Pa) is a component of central neural circuitry that regulates several homeostatic variables. The paraventricular nucleus is composed of magnocellular neurons that project to the posterior pituitary and parvicellular neurons that project to numerous sites in the central nervous system. According to the revised prosomeric model, the paraventricular nucleus is located caudal to the eye stalk along the rostrocaudal dimension of the dorsal hypothalamic alar plate. Caudally, the paraventricular nucleus abuts the prethalamus (prosomere 3), and the entire complex is flanked ventrally and dorsally by Dlx5-expressing domains of the alar plate. The homeodomain transcription factor Orthopedia (Otp) is expressed in several separate hypothalamic sites: the paraventricular nucleus, perimammillary region and arcuate nucleus. In this study, we compared Otp expression in the hypothalamus of mouse (Mus musculus), chick (Gallus gallus), frog (Rana perezi) and axolotol (Ambystoma mexicanum), using immunohistochemical and in situ hybridization techniques. In all cases, Otp-positive cells in the paraventricular nucleus were excluded from Dlx5-expressing adjacent domains. Other positive neuronal populations were observed in the arcuate nucleus and oblique perimammillary band. Expression in the medial amygdala appears to be continuous with the Otp-expressing paraventricular nucleus complex. This area is relatively unevaginated in the amphibian brains, barely evaginated in the chick, and fully evaginated in the mouse. These data led us to conclude that the expression pattern of Otp is topologically highly conserved in tetrapods and is plesiomorphic among chordates. (c) 2007 Elsevier Inc. All rights reserved.