TRANSFORMATION OF BIOCHEMICALLY DEFICIENT STRAINS OF BACILLUS-SUBTILIS BY DEOXYRIBONUCLEATE
TRANSFORMATION OF BIOCHEMICALLY DEFICIENT STRAINS OF BACILLUS-SUBTILIS BY DEOXYRIBONUCLEATE
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DOI：
10.1073/pnas.44.10.1072
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发表时间：
1958-01-01
影响因子：
11.1
通讯作者：
SPIZIZEN, J
中科院分区：
文献类型：
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作者：
SPIZIZEN, J
用从野生型B分离的脱氧核糖核酸（DNA）转化枯草芽孢杆菌的三个营养缺陷型突变体，使其营养不依赖。枯草杆菌，菌株23。只要2 × 10-4 [mu]g的DNA就能转化热处理过的孢子，使其在葡萄糖基本培养基中发芽。产生的转化体的数量与加入的DNA的量直接相关，并且通过用脱氧核糖核酸酶处理来防止转化。供体DNA的比活性通过去除蛋白质而增加，并且似乎与供体DNA密切相关的核糖核酸部分增强了转化活性。酵母提取物中的一种未鉴定的因子也是有效转化所必需的。有效的DNA在低离子强度的溶液中对冷冻和储存相对不稳定，但在2 [image] NaCl溶液中相对稳定。
Three auxotrophic mutants of Bacillus subtilis were transformed to nutritional independence with deoxyribonucleate (DNA) isolated from a wild type of B. subtilis, strain 23. As little as 2 x 10-4 [mu]g of DNA effects transformation of heat-treated spores allowed to germinate in a glucose-minimal medium. The number of transformants produced is directly related to the quantitity of DNA added and transformation is prevented by treatment with deoxyribonuclease. The specific activity of the donor DNA is increased by removal of protein and it also appears that a ribonucleate fraction, closely associated with donor DNA, enhances transformation activity. An unidentified factor in yeast extract is also required for efficient transformation. The effective DNA is relatively labile to freezing and storage in solutions of low ionic strength, but is relatively stable in 2 [image] NaCl solutions.