OsFIE2 plays an essential role in the regulation of rice vegetative and reproductive development
OsFIE2 plays an essential role in the regulation of rice vegetative and reproductive development
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DOI：
10.1111/nph.12472
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发表时间：
2014-01-01
期刊：
影响因子：
9.4
通讯作者：
Sun, Meng-Xiang
中科院分区：
文献类型：
--
作者：
Li, Shisheng;Zhou, Bing;Sun, Meng-Xiang
Polycomb group （PcG）蛋白是一种基因抑制因子，在发育过程中通过染色质重塑帮助维持细胞身份。受精不依赖胚乳（FIE）是PcG复合体的一员，在植物发育过程中起着广泛的作用，但迄今为止其在水稻中的作用尚未得到充分的研究。我们报道了水稻中PcG成员的分离和鉴定，并将其命名为Oryza sativa fertilisation - independent胚乳2的OsFIE2。OsFIE2是水稻基因组中的一个单拷贝基因，具有普遍的表达模式。OsFIE2 RNAi系在营养器官和生殖器官上均表现出多效性表型。在未受精的水稻中，胚乳的形成可以在没有胚胎形成的情况下被触发，这表明FIE确实参与了抑制水稻自主胚乳发育的过程。此外，OsFIE2 RNAi系的根中侧根的产生较早，而主根则是早熟且高度分化的。随着根尖干细胞的分化，静止中心维持干细胞所需的QHB基因下调。我们的数据表明，OsFIE2-PcG复合物对水稻繁殖和胚乳形成至关重要。它在干细胞维持中的作用表明，该基因在植物和动物中都是功能保守的。
Polycomb group (PcG) proteins are gene repressors that help to maintain cellular identity during development via chromatin remodeling. Fertilization-independent endosperm (FIE), a member of the PcG complex, operates extensively in plant development, but its role in rice has not been fully investigated to date. We report the isolation and characterization of a PcG member in rice, which was designated OsFIE2 for Oryza sativa Fertilization-Independent Endosperm 2. OsFIE2 is a single-copy gene in the rice genome and shows a universal expression pattern. The OsFIE2 RNAi lines displayed pleiotropic phenotypes in vegetative and reproductive organ generation. In unfertilized lines, endosperm formation could be triggered without embryo formation, which indicates that FIE is indeed involved in the suppression of autonomous endosperm development in rice. Furthermore, lateral root generation was promoted early in the roots of OsFIE2 RNAi lines, whereas the primary root was premature and highly differentiated. As the root tip stem cell differentiated, QHB, the gene required for stem cell maintenance in the quiescent center, was down-regulated. Our data suggest that the OsFIE2-PcG complex is vital for rice reproduction and endosperm formation. Its role in stem cell maintenance suggests that the gene is functionally conserved in plants as well as animals.