Xenopus Dead end mRNA is a localized maternal determinant that serves a conserved function in germ cell development
Xenopus Dead end mRNA is a localized maternal determinant that serves a conserved function in germ cell development
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DOI：
10.1016/j.ydbio.2005.06.013
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发表时间：
2006-03-01
影响因子：
2.7
通讯作者：
Pieler, T
中科院分区：
文献类型：
--
作者：
Horvay, K;Claussen, M;Pieler, T
种质形成被认为是生殖细胞发育的第一步。非洲爪蟾死端代表了一种种质特异性转录本，与先前表征的斑马鱼死端同源，这是生殖细胞迁移和存活所必需的。XDead end mRNA定位于非洲爪蟾卵母细胞的植物极;与非洲爪蟾中所有其他已知的胚质相关转录物不同，XDead end是通过晚期运输途径运输的，这表明存在不同的胚质限制模式。卵母细胞中的植物定位通过定位元件映射到3 '-UTR中的251个核苷酸元件来实现。该RNA序列与一组晚期定位途径的蛋白质特征和一种38 kDa的额外蛋白质结合。在非洲爪蟾胚胎中抑制XDead end翻译会导致蝌蚪发育阶段原始生殖细胞的损失。早期的规格事件似乎没有受到影响，但原始生殖细胞未能迁移到背侧，并最终消失。这种表型与在斑马鱼中观察到的非常相似，表明XDead end在哺乳动物细胞发育中的作用在进化中是保守的。(c)2005年爱思唯尔公司All rights reserved.
Germ plasm formation is considered to define the first step in germ cell development. Xenopus Dead end represents a germ plasm specific transcript that is homologous to the previously characterized zebrafish dead end, which is required for germ cell migration and survival. XDead end mRNA localizes to the vegetal pole of Xenopus oocytes; in contrast to all other known germ plasm associated transcripts in Xenopus, XDead end is transported via the late transport pathway, suggesting a different mode of genii plasm restriction. Vegetal localization in the oocyte is achieved via a localization element mapping to a 251 nucleotide element in the 3'-UTR. This RNA sequence binds to a set of proteins characteristic for the late localization pathway and to one additional protein of 38 kDa. Inhibition of XDead end translation in Xenopus embryos results in a loss of primordial germ cells at tadpole stages of development. Early specification events do not seem to be affected, but the primordial germ cells fail to migrate dorsally and eventually disappear. This phenotype is very similar to what has been observed in the zebrafish, indicating that the role of XDead end in genu cell development has been conserved in evolution. (c) 2005 Elsevier Inc. All rights reserved.