Bioassay of parathyroid hormone in vitro with a stable preparation of adenyl cyclase from rat kidney.
Bioassay of parathyroid hormone in vitro with a stable preparation of adenyl cyclase from rat kidney.
复制标题
使用来自大鼠肾脏的稳定的腺苷酸环化酶制剂对甲状旁腺激素进行体外生物测定。
作者：
Robert Marcus;G. Aurbach
基于大鼠肾皮质腺苷酸环化酶的激活，建立了甲状旁腺激素的体外生物测定法。在含有10%v/v二甲亚砜的0.05M Tris-HCl（pH 7.4）中制备酶，允许在液氮中储存超过30天，而对甲状旁腺激素或氟化物的敏感性没有显著损失。该测定法对低至0.14 USP U的甲状旁腺激素敏感，并在0.14 - 1.13 USP U的激素范围内显示出对数线性剂量响应。在一系列的23个测定的平均精密度指数为0.083。该测定法对甲状旁腺激素具有高度特异性，并在连续的纯化阶段显示出适当的激素制剂效价增量。体外腺苷酸环化酶法的结果与体内标准生物测定法的结果相似。其他激素和蛋白质，包括在粗甲状旁腺提取物中发现的非激素污染物没有显着干扰。那个...
A bioassay for parathyroid hormone in vitro was developed based upon the activation of adenyl cyclase from the renal cortex of the rat. Preparation of the enzyme in 0.05M Tris-HCl, pH 7.4, containing 10% v/v dimethylsulfoxide allowed storage in liquid nitrogen for periods in excess of 30 days without significant loss of sensitivity to parathyroid hormone or fluoride. The assay was sensitive to as little as 0.14 USP U of parathyroid hormone, and showed a log-linear dose response over the range of 0.14– 1.13 USP U of hormone. The average index of precision in a series of 23 assays was 0.083. Theassay was highly specific for parathyroid hormone and showed appropriate increments in potency for hormone preparations at consecutive stages of purification. Results with the adenyl cyclase method in vitro were similar to those obtained with a standard bioassay in vivo. There was no significant interference from other hormones and proteins, including nonhormonal contaminants found in crude parathyroid extracts. The ...