Biological function of unannotated transcription during the early development of Drosophila melanogaster
Biological function of unannotated transcription during the early development of Drosophila melanogaster
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DOI：
10.1038/ng1875
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发表时间：
2006-10-01
期刊：
影响因子：
30.8
通讯作者：
Gingeras, Thomas R.
中科院分区：
文献类型：
--
作者：
Manak, J. Robert;Dike, Sujit;Gingeras, Thomas R.
许多动物和植物基因组的转录范围比当前注释预测的要广泛得多。然而，这些未注释的转录区域的生物学功能在很大程度上是未知的。在黑腹果蝇胚胎发生过程中检测到的转录核苷酸中大约 7% 和 23% 分别映射到未注释的基因间和内含子区域。基于协调转录的计算分析，我们保守地估计所有未注释的转录序列中有 29% 作为已明确表征的蛋白质编码基因的缺失或替代外显子发挥作用。我们估计 15.6% 的基因间转录区域作为缺失或替代转录起始位点 (TSS)，被 11.4% 的表达蛋白质编码基因使用。新鉴定的 5' 外显子内或附近 P 元件突变的鉴定提供了将先前未表征的突变映射到其各自基因的策略。总的来说，这些数据表明至少 85% 的果蝇基因组被转录并加工成代表至少 30% 的果蝇基因组的成熟转录本。
Many animal and plant genomes are transcribed much more extensively than current annotations predict. However, the biological function of these unannotated transcribed regions is largely unknown. Approximately 7% and 23% of the detected transcribed nucleotides during D. melanogaster embryogenesis map to unannotated intergenic and intronic regions, respectively. Based on computational analysis of coordinated transcription, we conservatively estimate that 29% of all unannotated transcribed sequences function as missed or alternative exons of well-characterized protein-coding genes. We estimate that 15.6% of intergenic transcribed regions function as missed or alternative transcription start sites (TSS) used by 11.4% of the expressed protein-coding genes. Identification of P element mutations within or near newly identified 5' exons provides a strategy for mapping previously uncharacterized mutations to their respective genes. Collectively, these data indicate that at least 85% of the fly genome is transcribed and processed into mature transcripts representing at least 30% of the fly genome.